ASRGL1 Mouse mAb - Primärer Antikörper, specific to ASRGL1, Mouse IgG2a

Cat. No.: Ab089867

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG2a

Clone Number

K52014_9F2

Conjugation

Unconjugated

Übersicht

Mouse anti Human ASRGL1 Antibody, Monoclonal (K52014_9F2), could be used for WB, IHC and so on.

Specifications

Product Name

ASRGL1 Mouse mAb - Primärer Antikörper, specific to ASRGL1, Mouse IgG2a

Synonyme

ALP antibody | ALP1 antibody | ASGL1antibody | ASGL1_HUMAN antibody | Asparaginase like 1 antibody | Asparaginase like 1 protein antibody | Asparaginase like protein 1antibody | Asparaginase-like protein 1 antibody | ASRGL 1 antibody | Asrgl1 antibody | B

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Biochemische und physiologische Mechanismen

Has both L-asparaginase and beta-aspartyl peptidase activity. May be involved in the production of L-aspartate, which can act as an excitatory neurotransmitter in some brain regions. Is highly active with L-Asp beta-methyl ester. Besides, has catalytic ac

Wirtsspezies

Maus

Spezifität

ASRGL1

Immunogen

Recombinant protein of human ASRGL1 (AA 1-308).

Positive Kontrolle

WB: Hep G2 and Jurkat cell lysates. IHC: Human endometrium tissue.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG2a

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: ASRGL1 Mouse mAb (Ab089867)

ASRGL1 Mouse mAb (Ab089867) - Western Blot
All lanes: ASRGL1 Mouse mAb (Ab089867) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 32 kDa
Observed band size: 16, 38 kDa
ASRGL1 Mouse mAb (Ab089867) recognizes two isoforms of ASRGL1.
Exposure time: 60.0 s

IHC: ASRGL1 Mouse mAb (Ab089867)

ASRGL1 Mouse mAb (Ab089867) - IHC
IHC analysis of endometrium tissue by ASRGL1 Mouse mAb (Ab089867).
IHC was performed using sections of the formalin-fixed paraffin-embedded endometrium tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with ASRGL1 Mouse mAb (Ab089867) at 1 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Glandular cells are positively stained at the cytoplasm.

Anwendung

• Anwendung: Dilution info
• WB:

  1/1000 ;

• IHC:

  1/1000

Zugehörige Ziele (menschlich)

ASRGL1 Tbio Isoaspartyl peptidase/L-asparaginase (0 Activities)

Discover Target: ASRGL1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)