GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human ATG4D Antibody, Monoclonal (P2S15), could be used for WB, IF,ICC and so on.
Specifications
Product Name
ATG4D Mouse mAb - Primary antibody, specific to ATG4D, Mouse IgG1
Synonyms
APG4 autophagy 4 homolog D antibody | APG4 Dantibody | APG4, S. cerevisiae, homolog of, D antibody | APG4D antibody | ATG4 autophagy related 4 homolog D (S. cerevisiae) antibody | Atg4d antibody | ATG4D_HUMAN antibody | Atg4dl antibody | 9830134P12Rik ant
Specifications & Purity
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Biochemical and Physiological Mechanisms
Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a reveal
Host species
Mouse
Specificity
ATG4D
Immunogen
Recombinant fusion protein of human ATG4D.
Positive Control
WB: A431, A549, HeLa and NIH/3T3 cell lysates. IF/ICC: U251 cells.
Conjugation
Unconjugated
Grade
Carrier Free, ExactAb™, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
0.5 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
ATG4D Mouse mAb (Ab090017) - Western Blot All lanes: ATG4D Mouse mAb (Ab090017) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 52 kDa Observed band size: 45 kDa Exposure time: 100.0 s
ATG4D Mouse mAb (Ab090017) - IF/ICC Immunofluorescent analysis of ATG4D staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ATG4D Mouse mAb (Ab090017) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Application
Application
Dilution info
WB
1/1000 - 1/2000
IF/ICC
1/10 - 1/50
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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