ATG4D Mouse mAb - Primärer Antikörper, specific to ATG4D, Mouse IgG1

Cat. No.: Ab090017

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Maus

Reactive species

Maus, Mensch

Isotype

Mouse IgG1

Clone Number

P2S15

Conjugation

Unconjugated

Übersicht

Mouse anti Human ATG4D Antibody, Monoclonal (P2S15), could be used for WB, IF,ICC and so on.

Specifications

Product Name

ATG4D Mouse mAb - Primärer Antikörper, specific to ATG4D, Mouse IgG1

Synonyme

APG4 autophagy 4 homolog D antibody | APG4 Dantibody | APG4, S. cerevisiae, homolog of, D antibody | APG4D antibody | ATG4 autophagy related 4 homolog D (S. cerevisiae) antibody | Atg4d antibody | ATG4D_HUMAN antibody | Atg4dl antibody | 9830134P12Rik ant

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a reveal

Wirtsspezies

Maus

Spezifität

ATG4D

Immunogen

Recombinant fusion protein of human ATG4D.

Positive Kontrolle

WB: A431, A549, HeLa and NIH/3T3 cell lysates. IF/ICC: U251 cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: ATG4D Mouse mAb (Ab090017)

ATG4D Mouse mAb (Ab090017) - Western Blot
All lanes: ATG4D Mouse mAb (Ab090017) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 45 kDa
Exposure time: 100.0 s

IF/ICC: ATG4D Mouse mAb (Ab090017)

ATG4D Mouse mAb (Ab090017) - IF/ICC
Immunofluorescent analysis of ATG4D staining in U251 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ATG4D Mouse mAb (Ab090017) in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Anwendung

• Anwendung: Dilution info
• WB:

  1/1000 - 1/2000

• IF/ICC:

  1/10 - 1/50 ;

Zugehörige Ziele (menschlich)

ATG4D Tbio Cysteine protease ATG4D (0 Activities)

Discover Target: ATG4D

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)