BRAF Mouse mAb - Primärer Antikörper, specific to BRAF, Mouse IgG1

Cat. No.: Ab091608
Zu bestellen verfügbar
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 0.5 mg/mL
Application
IF/ICC, WB
Host species
Maus
Reactive species
Mensch
Isotype
Mouse IgG1
Clone Number
125CT13.3.5
Conjugation
Unconjugated
★
Size
Deutschland (EU)
USA*
Price
Qty
10μl
Ab091608-10μl
—
4 Auf Lager
69,33€
50μl
Ab091608-50μl
—
2 Auf Lager
225,53€
100μl
Ab091608-100μl
Auf Bestellung · 8–12 Wochen
364,36€
1ml
Ab091608-1ml
Auf Bestellung · 8–12 Wochen
2.950,23€
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, Carrier Free, 0.5 mg/mL Carrier Free,ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Übersicht

Mouse anti Human BRAF Antibody, Monoclonal (125CT13.3.5), could be used for WB, IF, ICC and so on.
Application:
WB: 1/500-1/2000
ICC/IF: 1/10-1/50
Protein Function
Involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron.

Specifications

Product Name
BRAF Mouse mAb - Primärer Antikörper, specific to BRAF, Mouse IgG1
Synonyme
FLJ95109 antibody | 94 kDa B raf protein antibody | B raf 1 antibody | B raf antibody | B Raf proto oncogene serine threonine protein kinase antibody | B Raf proto oncogene, serine/threonine kinase antibody | B RAF1 antibody | B-Raf proto-oncogene serine/
Spezifikationen & Reinheit
ExactAb™, Validated, Carrier Free, 0.5 mg/mL
Wirtsspezies
Maus
Spezifität
BRAF
Immunogen
BRAF recombinant protein (AA 4-365).
Positive Kontrolle
WB: MCF-7, K562, HeLa, Hep G2 and HEK293 cell lysates. ICC/IF: C2C12 cells
Konjugation
Unconjugated
Note
Carrier Free, ExactAb™, Validated
Isotyp
Mouse IgG1
Lichterketten-Typ
kappa
SDS-PAGE
150 kDa
Verfahren zur Reinigung
Protein G purified
Konzentration
0.5 mg/mL
Lagerung und Versand
Form
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Bilder

BRAF Mouse mAb (Ab091608) - Western Blot
All lanes: BRAF Mouse mAb (Ab091608) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 86 kDa
Observed band size: 85 kDa
Exposure time: 17.9 s

BRAF Mouse mAb (Ab091608) - ICC/IF
Fluorescent image of C2C12 cells stained with BRAF Antibody (Ab091608). C2C12 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with BRAF primary antibody 1:25, 1 h at 37℃. For secondary antibody, AF488 conjugated donkey anti-mouse antibody (green) was used 1:400, 50 min at 37℃. Cytoplasmic actin was counterstained with AF555 (red) conjugated Phalloidin 7 units/mL, 1 h at 37℃. BRAF immunoreactivity is localized to Cytoplasm significantly.

Anwendung
AnwendungDilution info
WB

1/500-1/2000

ICC/IF

1/10-1/50

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Zugehörige Ziele (menschlich)
BRAF Tclin Serine/threonine-protein kinase B-raf (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
BRAF Tclin Serine/threonine-protein kinase B-raf (11587 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
BRAF Tclin Protein cereblon/BRAF (4 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Wirkungsmechanismen
Zertifikate (CoA, COO, BSE/TSE und Analyse-Diagramm)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDatumArtikel
ZJ24F1011989Certificate of AnalysisAug 11, 2026 Ab091608
ZJ23F0800966Certificate of AnalysisSep 04, 2023 Ab091608
ZJ23F0800965Certificate of AnalysisSep 04, 2023 Ab091608
FAQs und Artikel
Lösungsrechner
Bewertungen

Kundenbewertungen

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide →

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