CD22 Mouse mAb - Primärer Antikörper, specific to CD22, Mouse IgG1

Cat. No.: Ab094278

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Depletion, Flow Cytometry, Functional Assay, IP

Host species

Maus

Reactive species

Maus

Isotype

Mouse IgG1

Clone Number

Cy34.1

Conjugation

Unconjugated

Übersicht

Mouse anti Mouse CD22 Antibody, Monoclonal (Cy34.1), could be used for in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5), Flow, IP and so on.
Application:
in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5): Use at an assay dependent concentration. 
Flow: Use at an assay dependent concentration.
IP: Use at an assay dependent concentration. 
Protein Function
Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2,6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.

Specifications

Product Name

CD22 Mouse mAb - Primärer Antikörper, specific to CD22, Mouse IgG1

Synonyme

Lyb8 | Lyb-8 | A530093D23 | B-cell receptor CD22 | BL-CAM | B-lymphocyte cell adhesion molecule | CD22 antigenMGC130020 | CD22 molecule | CD22 | sialic acid binding Ig-like lectin 2 | Sialic acid-binding Ig-like lectin 2 | Siglec2 | Siglec-2 | SIGLEC2FLJ2

Spezifikationen & Reinheit

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Wirtsspezies

Maus

Spezifität

CD22

Immunogen

B10.D2 mouse splenocytes

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Bilder

Flow Cytometry: CD22 Mouse mAb (Ab094278)

CD22 Mouse mAb (Ab094278) - Flow Cytometry
Two-color flow cytometric analysis of CD22 expression on mouse splenocytes. C57BL/6 mouse spleen cells were stained with CD3 Rat mAb (FITC) (Ab185406) and either Mouse IgG Isotype Control Antibody (Biotin) (Ab180306; Left panel) or CD22 Mouse mAb (Ab094278; Right panel) at 0.5 μg/mL. Two-color flow cytometric dot plots showing the correlated expression of CD3 versus CD22 (or Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable mononuclear cells.

Anwendung

• Anwendung: Dilution info
• IP:

  Use at an assay dependent concentration.

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo B cell depletion in combination with anti-CD19 (clone 1D3) and anti-rat κLight Chain (clone MAR 18.5):

  Use at an assay dependent concentration. ;