CD3 epsilon Mouse mAb - Primärer Antikörper, specific to CD3E, Mouse IgG1

Cat. No.: Ab094446

QUALITÄT & REINHEIT Carrier Free, ExactAb™, Validated, High Performance, See COA

Application

IHC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

C10

Conjugation

Unconjugated

Übersicht

Mouse anti Human CD3 epsilon Antibody, Monoclonal (C10), could be used for WB, IHC and so on.
Application:
WB: 0.01-3µg/mL
IHC: 5-20µg/mL
Protein Function:
The CD3 complex mediates signal transduction.

Spezifikationen

Product Name

CD3 epsilon Mouse mAb - Primärer Antikörper, specific to CD3E, Mouse IgG1

Synonyme

T cell surface antigen T3/Leu 4 epsilon chain antibody | T cell surface glycoprotein CD3 epsilon chain antibody | T-cell surface antigen T3/Leu-4 epsilon chain antibody | T-cell surface glycoprotein CD3 epsilon chain antibody | T3E antibody | TCRE antibod

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Validated, High Performance, See COA

Wirtsspezies

Maus

Spezifität

CD3E

Immunogen

Recombinant CD3e expressed in E.coli (AA 28-185)

Positive Kontrolle

WB: Jurkat and MOLT-4 cell lysates. IHC: Human spleen tissue.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, High Performance, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A + Protein G purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: CD3 epsilon Mouse mAb (Ab094446)

CD3 epsilon Mouse mAb (Ab094446) - Western Blot
All lanes: CD3 epsilon Mouse mAb (Ab094446) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 22 kDa
Exposure time: 20.0 s

IHC: CD3 epsilon Mouse mAb (Ab094446)

CD3 epsilon Mouse mAb (Ab094446) - IHC
DAB staining on IHC
Sample: Human Spleen Tissue
Primary Ab: 20 µg/mL CD3 epsilon Mouse mAb (Ab094446)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Anwendung

Anwendung

Dilution info

WB

0.01-3 µg/mL

IHC

5-20 µg/mL

Zugehörige Ziele (menschlich)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD3E Tclin T-cell surface glycoprotein CD3 epsilon chain (0 Activities)

Discover Target: CD3E

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD3 epsilon Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.