CD4 Mouse mAb (APC) - Primärer Antikörper, specific to CD4, Mouse IgG2b

Cat. No.: Ab180133

GRADE & PURITY Validated, Ex:650nm, Em:660nm, 5 μL/test

Application

Flow Cytometry

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG2b

Clone Number

OKT4

Conjugation

APC

Übersicht

Application:
Flow: 5 μL/test
For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

Specifications

Product Name

CD4 Mouse mAb (APC) - Primärer Antikörper, specific to CD4, Mouse IgG2b

Synonyme

CD_antigen: CD4 | CD4 antigen (p55) | CD4 antigen | CD4 molecule | CD4 receptor | CD4 | CD4mut | T-cell surface antigen T4/Leu-3 | T-cell surface glycoprotein CD4 | CD 4 antibody | CD4 (L3T4) antibody | CD4 antibody | CD4 antigen (p55) antibody | CD4 anti

Spezifikationen & Reinheit

Validated, Ex:650nm, Em:660nm, 5 μL/test

Wirtsspezies

Maus

Spezifität

CD4

Immunogen

Human peripheral T cells.

Positive Kontrolle

Flow: Human peripheral blood lymphocytes

Konjugation

APC

Anregung (nm)

Red Laser(633nm)

Emission (nm)

660nm

Ex/Em(nm)

Ex:650nm;Em:660nm

Note

Em:660nm, Ex:650nm, Validated

Isotyp

Mouse IgG2b

Verfahren zur Reinigung

Protein G purified

Konzentration

5 μL/test

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Protected from light,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Bilder

Flow Cytometry: CD4 Mouse mAb (APC) (Ab180133)

CD4 Mouse mAb (APC) (Ab180133) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD4 Mouse mAb (APC) (Ab180133) at 5μL/test for 1 hour at 4°C. Left panel - Mouse IgG Isotype Control Antibody (APC) (Ab180309).

Flow Cytometry: CD4 Mouse mAb (APC) (Ab180133)

CD4 Mouse mAb (APC) (Ab180133) - Flow Cytometry
Flow cytometry analysis of CD4 expression by Human peripheral blood lymphocytes (red) compared to an isotype control (blue). Cells were stained with CD4 Mouse mAb (APC) (Ab180133) at 5μL/test for 1 hour at 4°C. Blue - Mouse IgG Isotype Control Antibody (APC) (Ab180309). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Anwendung

Dilution info

Flow Cytometry

5μl/test

For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. 

Zugehörige Ziele (menschlich)

CD4 Tclin T-cell surface glycoprotein CD4 (0 Activities)

Discover Target: CD4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

How should this product be stored?

Store at 2–8 °C, protected from light. Do not freeze. Freezing may cause irreversible damage to this material.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Ex:650nm indicate?

Ex:650nm is a product attribute used to filter and describe certain reagents in our catalog, rather than a standalone grade with its own specifications. It is most commonly used to identify products suitable for fluorescent labeling and detection.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (CD4 Mouse mAb (APC)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.