CD99 Mouse mAb - Primärer Antikörper, specific to CD99, Mouse IgG1

Cat. No.: Ab006798

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

Flow Cytometry, IHC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

1B10E12

Conjugation

Unconjugated

Übersicht

Mouse anti Human CD99 Antibody, Monoclonal, could be used for WB, IHC, Flow Cytometry and so on.

Specifications

Product Name

CD99 Mouse mAb - Primärer Antikörper, specific to CD99, Mouse IgG1

Synonyme

12E7 | E2 antigen | Protein MIC2 | T-cell surface glycoprotein E2

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0mg/mL

Biochemische und physiologische Mechanismen

Involved in T-cell adhesion processes. It is involved in spontaneous rosette formation with erythrocytes. Post-translational: Extensively O-glycosylated.

Wirtsspezies

Maus

Spezifität

CD99

Immunogen

Purified recombinant fragment of human CD99 (AA: 1-185) expressed in E. Coli.

Positive Kontrolle

WB: MOLT-4, Jurkat lysates. IHC: Human bladder cancer, ewing sarcoma tissues. Flow Cytometry: Jurkat cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

1.0mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (36 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: CD99 Mouse mAb (Ab006798)

CD99 Mouse mAb (Ab006798) - Western Blot
All lanes: CD99 Mouse mAb (Ab006798) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution

Predicted band size: 19 kDa
Observed band size: 28&32 kDa

Flow Cytometry: CD99 Mouse mAb (Ab006798)

CD99 Mouse mAb (Ab006798) - Flow Cytometry
Flow cytometric analysis of Jurkat cells using CD99 Mouse mAb (Ab006798) (green) and negative control (red).

IHC: CD99 Mouse mAb (Ab006798)

CD99 Mouse mAb (Ab006798) - IHC
Immunohistochemical analysis of paraffin-embedded human bladder cancer tissues using CD99 mouse mAb (Ab006798) with DAB staining.

IHC: CD99 Mouse mAb (Ab006798)

CD99 Mouse mAb (Ab006798) - IHC
Immunohistochemical analysis of paraffin-embedded human ewing sarcoma tissues using CD99 (Ab006798) mouse mAb with DAB staining.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• ELISA:

  1/10000 ;

• IHC:

  1/200-1/1000 ;

• Flow Cytometry:

  1/200-1/400 ;

Zugehörige Ziele (menschlich)

CD99 Tbio CD99 antigen (0 Activities)

Discover Target: CD99

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)