Coatomer subunit delta/COPD Mouse mAb - Primärer Antikörper, specific to ARCN1

Cat. No.: Ab097079

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Maus

Reactive species

Affe, Allerdings, Maus, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

OTI6A6

Conjugation

Unconjugated

Übersicht

Mouse anti Human Coatomer subunit delta/COPD Antibody, Monoclonal (OTI6A6), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

Coatomer subunit delta/COPD Mouse mAb - Primärer Antikörper, specific to ARCN1

Synonyme

Archain 1 antibody | Archain antibody | Archain vesicle transport protein 1 antibody | Arcn1 antibody | Coatomer delta subunit antibody | Coatomer protein complex, subunit delta antibody | Coatomer protein delta COP antibody | Coatomer subunit delta antib

Spezifikationen & Reinheit

ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coa

Wirtsspezies

Maus

Spezifität

ARCN1

Immunogen

Recombinant full length protein of human Coatomer subunit delta/COPD.

Positive Kontrolle

WB: Hep G2, HeLa and HEK293 cell lysates. IHC: Human colon adenocarcinoma, pancreas, endometrium adenocarcinoma, prostate carcinoma tissues. IF/ICC: COS7 cells transiently transfected with pCMV6-ENTRY ARCN1. Flow Cytometry: HEK293T cells transfected with

Konjugation

Unconjugated

Note

ExactAb™, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - Western Blot
All lanes: Coatomer subunit delta/COPD Mouse mAb (Ab097079) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 60 kDa
Exposure time: 54.2 s

IHC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human colon tissue using Coatomer subunit delta/COPD Mouse mAb (Ab097079). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using Coatomer subunit delta/COPD Mouse mAb (Ab097079). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of Human endometrium tissue using Coatomer subunit delta/COPD Mouse mAb (Ab097079). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IHC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IHC
Immunohistochemical staining of paraffinembedded Carcinoma of Human prostate tissue using Coatomer subunit delta/COPD Mouse mAb (Ab097079). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min)

IF/ICC: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - IF/ICC
Coatomer subunit delta/COPD Mouse mAb (Ab097079) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARCN1 .

Flow Cytometry: Coatomer subunit delta/COPD Mouse mAb (Ab097079)

Coatomer subunit delta/COPD Mouse mAb (Ab097079) - Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by Coatomer subunit delta/COPD Mouse mAb (Ab097079), and then analyzed by flow cytometry.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

• IHC:

  1/50

Zugehörige Ziele (menschlich)

ARCN1 Tbio Coatomer subunit delta (0 Activities)

Discover Target: ARCN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)