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BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Pectin is a major component of the primary cell wall and middle lamella, primarily composed of protopectin, pectinic acid, and pectic acid. It contains monosaccharides such as galacturonic acid, lactose, arabinose, and glucuronic acid. As the most abundant polysaccharide in the cell walls of many higher plants, its unique physicochemical properties directly influence the texture and quality of plant-derived foods. Pectin molecules are cross-linked through Ca²⁺ bridges, as well as other ionic bonds, hydrogen bonds, glycosidic bonds, ester bonds, and benzene ring couplings. Based on different extraction methods, pectin can be classified into water-soluble pectin (WSP), ionically bound pectin (ISP), and covalently bound pectin (CSP). This kit employs an alkaline solution containing a chelating agent to extract covalently bound pectin (CSP) and quantifies it using the carbazole colorimetric method: Pectin is hydrolyzed to generate galacturonic acid, which undergoes a condensation reaction with carbazole reagent in a sulfuric acid solution to form a colored product with a maximum absorbance peak at 530 nm. By measuring the absorbance, the CSP content in the sample can be quantitatively calculated.
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