Crystal Violet Aqueous Solution (0.5%) - BioReagent,Biological Stain,for microscopy,0.5%

Cat. No.: C774833

GRADE & PURITY BioReagent, Biological Stain, for Microscopy, 0.5%

Storage

Protected from light,Room temperature

Shipped In

Normal

Application

Cell Staining

Overview

Crystal Violet, also known as Gentian Violet, is a basic dye soluble in water and ethanol, which stains tissues and cell nuclei purple. It is an excellent staining agent mainly used in cytology, histology, bacteriology, and other fields.

Protocol

I. Sample Preparation

1. For Paraffin Sections

(1) Deparaffinize in xylene for 5–10 min.

(2) Replace with fresh xylene and deparaffinize for another 5–10 min; treat with absolute ethanol for 5 min.

(3) 90% ethanol for 2 min; 70% ethanol for 2 min; distilled water for 2 min.

2. For Frozen Sections

Allow sections to equilibrate to room temperature, then rinse with distilled water for 2 min.

3. For Cultured Cells

(1) Fix with 4% paraformaldehyde for ≥10 min (or follow this alternative: add 5 mL of PBS/methanol solution per 25 cm², incubate for 2 min, discard the solution; add 5 mL of fresh methanol, incubate for 10 min, discard the solution).

(2) Rinse with distilled water for 2 min.(3) Replace with fresh distilled water and rinse for another 2 min.

II. Crystal Violet Staining

1. Stain with 0.5% Crystal Violet Aqueous Solution for 10 min (adjust time according to staining results and requirements).

2. Wash thoroughly with distilled or tap water, then observe and photograph.

Staining Result

Sample Type	Color
Tissues or Cells	Deep Purple

Precautions

1. Crystal Violet is harmful to humans; please take appropriate protective measures.

2. It is recommended to perform a pilot experiment on 1–2 samples when using this reagent for the first time.

3. For your safety and health, please wear a lab coat and disposable gloves during operation.

Specifications

Specifications & Purity

BioReagent, Biological Stain, for microscopy, 0.5%

Stability And Storage

Store at room temperature long term (12 months). Store in the dark.

Storage

Protected from light, Room temperature

Shipped In

Normal

Grade

Biological Stain, BioReagent, for Microscopy

Chemical and Physical Properties

• Sensitivity: Light-sensitive