EIF2S1 Mouse mAb - Primärer Antikörper, Mouse IgG1

Cat. No.: Ab101289

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

IF/ICC, WB

Host species

Maus

Reactive species

Maus, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

2E4F5E7

Conjugation

Unconjugated

Übersicht

Mouse anti Human EIF2S1 Antibody, Monoclonal (2E4F5E7), could be used for WB, IF/ICC and so on.

Specifications

Product Name

EIF2S1 Mouse mAb - Primärer Antikörper, Mouse IgG1

Synonyme

Eukaryotic translation initiation factor 2 subunit 1 | eIF-2-alpha | eIF-2A | EIF2A | CDA 02 | CDA02 | eIF 2 alpha | EIF 2 | EIF 2A | EIF-2alpha | EIF2 | EIF2alpha | Eukaryotic Translation Initiation Factor 2 alpha | IF2A_HUMAN antibody | Eukaryotic trans

Spezifikationen & Reinheit

ExactAb™, Validated, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA. This complex binds to a 40S ribosomal subunit, followed by mRNA binding to form a 43S preinitiation complex. Junction of the 60S ribosomal subunit

Wirtsspezies

Maus

Immunogen

Purified recombinant human eIF2α protein fragments expressed in E.coli.

Positive Kontrolle

WB: K562, HeLa, MCF7,C2C12, Hep G2, NIH/3T3 and C6 cell lysates. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

ExactAb™, Validated

Isotyp

Mouse IgG1

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: EIF2S1 Mouse mAb (Ab101289)

EIF2S1 Mouse mAb (Ab101289) - Western Blot
All lanes: EIF2S1 Mouse mAb (Ab101289) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 36 kDa
Observed band size: 35 kDa
Exposure time: 10.0 s

IF/ICC: EIF2S1 Mouse mAb (Ab101289)

EIF2S1 Mouse mAb (Ab101289) - IF/ICC
Immunofluorescent analysis of EIF2S1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with EIF2S1 Mouse mAb (Ab101289) in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000 ;

• IF/ICC:

  1/50-1/100

Zugehörige Ziele (menschlich)

EIF2S1 Tchem Eukaryotic translation initiation factor 2 subunit 1 (0 Activities)

Discover Target: EIF2S1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen