GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Pla
Wirtsspezies
Maus
Spezifität
EpCAM
Immunogen
Recombinant protein corresponding to Human EpCAM
Positive Kontrolle
WB: HT-29 whole cell lysate IF/ICC: HT-29 cells
Konjugation
Unconjugated
Note
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotyp
Mouse IgG1
Lichterketten-Typ
kappa
SDS-PAGE
150.0 KDa
Verfahren zur Reinigung
Protein A purified
Konzentration
1.0 mg/mL
Lagerung und Versand
Storage
Store at -20°C,Avoid repeated freezing and thawing
Verschickt in
Ice chest + Ice pads
Stabilität und Lagerung
Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Bilder
EpCAM Mouse mAb (Ab191888) - Western Blot All lanes: EpCAM Mouse Antibody (Ab191888) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 20 kDa Observed band size: 32, 39 kDa Exposure time: 60.0 s
EpCAM Mouse mAb (Ab191888) - ICC/IF IF analysis of EpCAM (Green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with EpCAM Mouse mAb (Ab191888) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.
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