EXOSC10/RRP6 Mouse mAb - Primärer Antikörper, specific to EXOSC10/RRP6, Mouse IgG1

Cat. No.: Ab102491

QUALITÄT & REINHEIT Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

K29029_1D2

Conjugation

Unconjugated

Übersicht

Mouse anti Human EXOSC10/RRP6 Antibody, Monoclonal (K29029_1D2), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/200
IP: 1/100
Protein Function:
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. EXOSC10 has 3'-5' exonuclease activity (By similarity). EXOSC10 is required for nucleolar localization of C1D and probably mediates the association of SKIV2L2, C1D and MPP6 wth the RNA exosome involved in the maturation of 5.8S rRNA.

Spezifikationen

Product Name

EXOSC10/RRP6 Mouse mAb - Primärer Antikörper, specific to EXOSC10/RRP6, Mouse IgG1

Synonyme

Autoantigen PM/Scl 2 antibody | Exosc10 antibody | Exosome component 10 antibody | EXOSX_HUMAN antibody | P100 polymyositis scleroderma overlap syndrome associated autoantigen antibody | P100 polymyositis-scleroderma overlap syndrome-associated autoantige

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Wirtsspezies

Maus

Spezifität

EXOSC10/RRP6

Immunogen

Recombinant human EXOSC10 (AA 691-850).

Positive Kontrolle

WB: Raji, Jurkat, HeLa and A549 cell lysates. IHC: Human skin tissue. IP: HEK293 lysate.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: EXOSC10/RRP6 Mouse mAb (Ab102491)

EXOSC10/RRP6 Mouse mAb (Ab102491) - Western Blot
All lanes: EXOSC10/RRP6 Mouse mAb (Ab102491) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/40000 dilution

Predicted band size: 101 kDa
Observed band size: 101 kDa
Exposure time: 150.0 s

IHC: EXOSC10/RRP6 Mouse mAb (Ab102491)

EXOSC10/RRP6 Mouse mAb (Ab102491) - IHC
IHC-P analysis of skin tissue by EXOSC10/RRP6 Mouse mAb (Ab102491).
IHC-P was performed using sections of the formalin-fixed paraffin-embedded skin tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9.0 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with EXOSC10/RRP6 Mouse mAb (Ab102491) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.
Result: Cells in epidermis are positively stained at the nuclei.

IP: EXOSC10/RRP6 Mouse mAb (Ab102491)

EXOSC10/RRP6 Mouse mAb (Ab102491) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of EXOSC10/RRP6 Mouse mAb (Ab102491) with HEK293 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. EXOSC10 at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody.
Lane 1: HEK293 lysate
Lane 2: EXOSC10 immunoprecipitated from HEK293 lysate by EXOSC10/RRP6 Mouse mAb (Ab102491) 
Lane 3: The isotype control antibody instead of EXOSC10/RRP6 Mouse mAb (Ab102491) in HEK293 lysate 
Result: EXOSC10/RRP6 Mouse mAb (Ab102491) can immunoprecipitate EXOSC10.

Anwendung

Anwendung

Dilution info

WB

1/1000

IP

1/100

IHC

1/200

Zugehörige Ziele (menschlich)

EXOSC10 Tbio Exosome component 10 (0 Activities)

Discover Target: EXOSC10

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (EXOSC10/RRP6 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.