ADSS1 Mouse mAb: FAQs, Specs & Usage Guide

ADSS1 Mouse mAb is a mouse-derived monoclonal antibody targeting adenylosuccinate synthetase isozyme 1 (ADSS1), supplied by Aladdin Scientific (SKU Ab350170) in PBS buffer for use in standard immunological research applications.

SKU / Catalog Number

Ab350170

Antibody Type

Mouse Monoclonal Antibody (mAb)

Target Protein

ADSS1 (Adenylosuccinate Synthetase Isozyme 1)

Formulation / Buffer

PBS (Phosphate-Buffered Saline) only

Clone Reference

absea #KC-34957

Host Species

Mouse

What Is ADSS1 and Why Is It Studied?

ADSS1, or adenylosuccinate synthetase isozyme 1, is an enzyme involved in the de novo purine biosynthesis pathway. It catalyzes the first committed step in the conversion of inosine monophosphate (IMP) to adenosine monophosphate (AMP) by condensing IMP with aspartate in a GTP-dependent reaction. ADSS1 is predominantly expressed in muscle tissue and plays a role in energy homeostasis and nucleotide metabolism. Research interest in ADSS1 spans metabolic diseases, muscle physiology, and emerging oncology studies, where purine metabolism is increasingly recognized as relevant to tumor cell proliferation.

Product Specifications for Ab350170

The ADSS1 Mouse mAb (Catalog No. Ab350170) is a mouse-derived monoclonal antibody sourced from absea (clone reference KC-34957). The antibody is formulated in PBS (phosphate-buffered saline) with no additional carrier proteins or preservatives stated in the current product record. Researchers should consult the full datasheet available on the Aladdin Scientific product page for confirmed information on host species reactivity, immunogen sequence, molecular weight of the target, and recommended working concentrations for each validated application.

Key specifications at a glance:

• Catalog No.: Ab350170
• Antibody format: Mouse monoclonal (IgG class, pending datasheet confirmation)
• Buffer: PBS
• Clone: absea #KC-34957

Recommended Storage and Handling

Proper storage is essential for maintaining the activity and specificity of any monoclonal antibody. As a general guideline for PBS-formulated mouse monoclonal antibodies, the following practices are recommended (verify with the official datasheet for Ab350170-specific instructions):

• Short-term storage (up to 1 month): Store at 4 °C. Avoid repeated freeze-thaw cycles.
• Long-term storage: Aliquot and store at −20 °C or −80 °C. Adding a carrier protein such as BSA (0.1–1%) may improve stability if not already present in the formulation.
• Handling: Keep on ice during experiments. Avoid exposure to sodium azide if the antibody is to be used in live-cell assays.
• Sterility: Work under aseptic conditions when aliquoting to prevent microbial contamination.

Always refer to the certificate of analysis (CoA) and product datasheet for lot-specific storage recommendations.

Typical Applications and Protocol Tips

Mouse monoclonal antibodies against metabolic enzymes such as ADSS1 are commonly employed in the following immunological techniques. Specific application validation status for Ab350170 should be confirmed on the Aladdin Scientific datasheet:

• Western Blot (WB): Denature samples under reducing conditions. Use standard SDS-PAGE appropriate for the predicted molecular weight of ADSS1 (~50 kDa; verify on datasheet). Block with 5% non-fat milk or BSA in TBST. Incubate primary antibody overnight at 4 °C at the recommended dilution.
• Immunoprecipitation (IP): Pre-clear lysates before adding antibody. Use protein A/G beads for capture of mouse IgG.
• Immunofluorescence (IF) / Immunohistochemistry (IHC): Optimize antigen retrieval method and antibody dilution empirically for tissue type. Use appropriate secondary anti-mouse IgG conjugate.
• ELISA: May be suitable for capture or detection depending on epitope; confirm with datasheet.

Always run a negative control (isotype control mouse IgG) in parallel to assess non-specific binding.

Ordering and Customer Support

ADSS1 Mouse mAb is available from Aladdin Scientific under catalog number Ab350170. To place an order or request a quote:

• Visit the Aladdin Scientific website and search for Ab350170 or ADSS1 Mouse mAb.
• Contact your regional Aladdin Scientific sales representative for bulk pricing, custom formulation inquiries, or institutional purchase orders.
• For technical questions about applications, cross-reactivity, or protocol optimization, reach out to the Aladdin Scientific technical support team.
• Certificates of Analysis (CoA) and Safety Data Sheets (SDS) can be requested by lot number via the website or customer service.

Returns and refunds are handled according to Aladdin Scientific's standard returns policy. Please inspect shipments upon arrival and report any quality concerns within the specified notification window.

Frequently asked questions

What is the buffer formulation of ADSS1 Mouse mAb (Ab350170)?

ADSS1 Mouse mAb (Ab350170) is formulated in PBS (phosphate-buffered saline) only. The current product information does not indicate additional stabilizers, carrier proteins, or preservatives. Researchers requiring a specific formulation should contact Aladdin Scientific customer support.

Which species does ADSS1 Mouse mAb (Ab350170) react with?

The species reactivity of Ab350170 is not specified in the current product record provided. Researchers should consult the full product datasheet on the Aladdin Scientific website or contact technical support to confirm whether human, mouse, rat, or other species reactivity has been validated.

What applications has ADSS1 Mouse mAb been validated for?

The validated applications for Ab350170 are not detailed in the current product record. Mouse monoclonal antibodies targeting metabolic enzymes like ADSS1 are typically tested for Western blot, immunoprecipitation, immunofluorescence, and immunohistochemistry, but confirmed application suitability must be verified in the official datasheet or by contacting Aladdin Scientific technical support.

How should I store ADSS1 Mouse mAb to maintain its activity?

As a general guideline for PBS-formulated monoclonal antibodies, store at 4 °C for short-term use (up to one month) and aliquot for storage at −20 °C or −80 °C for long-term preservation. Avoid repeated freeze-thaw cycles. Always confirm storage conditions with the lot-specific certificate of analysis (CoA) for Ab350170.

How do I place an order for Ab350170 from Aladdin Scientific?

You can order Ab350170 directly through the Aladdin Scientific website by searching the catalog number, or by contacting your regional sales representative or customer service team. Institutional purchase orders, bulk pricing, and custom requirements can also be accommodated through direct contact with Aladdin Scientific.

References

1. absea Biotechnology. Clone KC-34957 product information. absea internal reference.
2. Aladdin Scientific. ADSS1 Mouse mAb, Catalog No. Ab350170, product datasheet.
3. Stayton MM, Rudolph FB, Fromm HJ. Regulation, genetics, and properties of adenylosuccinate synthetase: a review. Curr Top Cell Regul. 1983;22:103-141.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.