PRRX1 Antibody: FAQs, Applications & Key Facts

PRRX1 Antibody is a research-grade immunological reagent designed to detect the paired related homeobox 1 (PRRX1) transcription factor, supporting applications such as Western blot and immunofluorescence in cell and developmental biology studies.

Product Name

PRRX1 Antibody

SKU / Catalog Number

Ab349221

Target Protein

PRRX1 (Paired Related Homeobox 1)

Protein Function

PRRX1 is a homeodomain transcription factor involved in limb development, mesenchymal differentiation, and epithelial-mesenchymal transition (EMT).

Supplier Reference

absea#OC-284

Recommended Storage

Store at -20°C; avoid repeated freeze-thaw cycles (general antibody storage guideline — verify with datasheet).

What Is PRRX1 and Why Is It an Important Research Target?

PRRX1 (Paired Related Homeobox 1), also known as PRX-1 or MHox, is a member of the paired-type homeodomain family of transcription factors. It plays critical roles in embryonic development, particularly in limb morphogenesis and craniofacial patterning. Beyond development, PRRX1 has gained significant attention in cancer biology as a regulator of epithelial-mesenchymal transition (EMT), a process associated with tumor invasion and metastasis.

Researchers studying stem cell biology, developmental biology, fibrosis, and oncology frequently require reliable tools to detect and quantify PRRX1 expression at the protein level. The PRRX1 Antibody (Ab349221) provides a validated immunological means to investigate these biological contexts.

Applications and Experimental Protocols for PRRX1 Antibody

The PRRX1 Antibody (Ab349221) is intended for use in standard immunodetection workflows. Typical applications for antibodies targeting nuclear transcription factors such as PRRX1 include:

• Western Blot (WB): Detect PRRX1 protein in whole-cell lysates or nuclear fractions. Because PRRX1 is a nuclear protein, nuclear extraction protocols are recommended to enrich the target and improve signal-to-noise ratio.
• Immunofluorescence (IF): Visualize subcellular localization of PRRX1 in fixed cells or tissue sections using fluorescence microscopy.
• Immunohistochemistry (IHC): Assess PRRX1 expression in formalin-fixed, paraffin-embedded (FFPE) tissue sections for translational and pathological studies.

Note: Confirmed application data, species reactivity, and recommended dilutions should be verified against the official product datasheet (absea#OC-284) prior to experimental use.

Storage, Handling, and Troubleshooting Tips

Storage: Antibodies are generally stored at -20°C in a non-frost-free freezer. Aliquoting the antibody upon first use is strongly recommended to minimize freeze-thaw cycles, which can degrade antibody performance over time. For short-term use (up to 1 month), storage at 4°C with sodium azide as a preservative is typical. Always consult the specific product datasheet for confirmed storage conditions for Ab349221.

Handling: Centrifuge the vial briefly before opening to consolidate the contents. Prepare working dilutions fresh on the day of the experiment. Avoid protein contamination by using clean pipette tips.

Troubleshooting: If background is high in Western blot, consider increasing blocking time (5% non-fat dry milk or BSA in TBST), optimizing primary antibody dilution, or extending wash steps. For weak or absent signal, verify cell/tissue expression of PRRX1 in the model used, check secondary antibody compatibility, and confirm sample preparation preserves nuclear proteins.

Ordering, Shipping, and Returns for Ab349221

Ordering: PRRX1 Antibody (Ab349221) can be ordered directly through the Aladdin Scientific website or by contacting our sales team. Bulk or custom quantity inquiries are welcome via our procurement channels.

Shipping: Antibodies are shipped on dry ice or with cold packs to maintain the cold chain during transit. Delivery timelines vary by destination; please contact customer service for estimated lead times in your region.

Returns and Refunds: If a product is received damaged or does not perform as described in the datasheet under recommended conditions, please contact Aladdin Scientific customer support within the applicable return window. Documentation such as lot number, experimental conditions, and images of results may be requested to process a return or replacement efficiently.

Frequently asked questions

What species does the PRRX1 Antibody (Ab349221) react with?

Confirmed species reactivity for Ab349221 should be verified directly in the official product datasheet (absea#OC-284). Typically, antibodies targeting PRRX1 are validated against human and may cross-react with mouse or rat due to sequence conservation, but this must be confirmed before experimental use.

What is the recommended dilution for PRRX1 Antibody in Western blot?

Recommended dilutions are specified in the product datasheet (absea#OC-284) and may vary by application. As a general starting point for transcription factor antibodies in Western blot, dilutions between 1:500 and 1:2000 are common, but empirical optimization in your specific cell or tissue system is always advised.

How should I prepare samples to detect PRRX1 by Western blot?

Since PRRX1 is a nuclear transcription factor, nuclear fractionation is recommended to enrich PRRX1 and improve detection sensitivity. Whole-cell lysis with RIPA buffer supplemented with protease inhibitors is an acceptable alternative, though signal may be diluted by cytoplasmic proteins. Ensure samples are heated in reducing SDS loading buffer prior to gel electrophoresis.

Can the PRRX1 Antibody be used for immunoprecipitation (IP) or ChIP assays?

The suitability of Ab349221 for immunoprecipitation or chromatin immunoprecipitation (ChIP) is not confirmed in the available source material. Please consult the product datasheet or contact Aladdin Scientific technical support to inquire about validated applications beyond WB, IF, and IHC.

How do I place an order for PRRX1 Antibody (Ab349221) and what payment options are available?

Ab349221 can be ordered via the Aladdin Scientific website, by email, or through your institution's procurement system. Accepted payment methods, pricing, and promotions are detailed on the Aladdin Scientific website; institutional purchase orders, credit cards, and other options are typically supported. Contact our sales team for quote or bulk pricing inquiries.

References

1. Absea Biotechnology product reference OC-284 (PRRX1 Antibody datasheet). Aladdin Scientific catalog Ab349221.
2. Bürglin TR, Affolter M. Homeodomain proteins: an update. Chromosoma. 2016;125(3):497-521.
3. Ocaña OH, et al. Metastatic colonization requires the repression of the epithelial-mesenchymal transition inducer Prrx1. Cancer Cell. 2012;22(6):709-724.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.