Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The gut microbiota can use dietary components, host secretions, and intrinsic microbial metabolic pathways to generate diverse classes of small-molecule compounds. These metabolites regulate the intestinal epithelial barrier, energy metabolism, immune-cell differentiation, and signaling in ...
HMGB1 converts cellular damage into inflammatory and chemotactic signals and regulates tumor immunity, tumor stemness, and therapy tolerance through TLRs, RAGE, and CXCR4.
The CD161-LLT1 axis connects NK cells, T cells, and the tumor microenvironment, and is one of the important pathways for evaluating tumor immune regulation and immune escape. Research on this axis should not remain limited to detecting CD161 or LLT1 expression levels alone. It should also ...
The extracellular matrix (ECM) in the tumor microenvironment not only supports tissue structure, but also continuously regulates immune cell infiltration, migration, activation, and effector functions through matrix deposition, crosslinking, degradation, and spatial reorganization. ECM ...
The BAFF pathway and the PD-1 pathway represent two typical directions in immune regulation by therapeutic monoclonal antibodies. The BAFF pathway mainly regulates B-cell survival, maturation, and autoantibody production, making it suitable for studies related to autoimmunity and abnormal ...
Techniques often explored alongside immunological experiments.
