Fibrinogen beta chain Mouse mAb - Primärer Antikörper, specific to Fibrinogen beta chain, Mouse IgG2b

Cat. No.: Ab103488

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

IHC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG2b

Clone Number

2#

Conjugation

Unconjugated

Übersicht

Mouse anti Human Fibrinogen beta chain Antibody, Monoclonal (2#), could be used for WB, IHC and so on.
Application
WB: 0.01-2 µg/mL
IHC: 5-20 μg/mL
Protein Function
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Specifications

Product Name

Fibrinogen beta chain Mouse mAb - Primärer Antikörper, specific to Fibrinogen beta chain, Mouse IgG2b

Synonyme

HEL-S-78p | Factor I | Fib2 | fibrinogen alpha chain | Fibrinogen | fibrinogen, A alpha polypeptide | MGC119422 | MGC119423 | MGC119425 | Beta fibrinogen antibody | Epididymis Secretory Sperm Binding Protein Li 78p antibody | FGB antibody | FIBB_HUMAN ant

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Wirtsspezies

Maus

Spezifität

Fibrinogen beta chain

Immunogen

Recombinant FGB expressed in E.coli (AA 45-491).

Positive Kontrolle

WB: Human serum and Hep G2 lysates. IHC: Human kidney, liver and placenta tissues.

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotyp

Mouse IgG2b

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A + Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Fibrinogen beta chain Mouse mAb (Ab103488)

Fibrinogen beta chain Mouse mAb (Ab103488) - Western Blot
All lanes: Fibrinogen beta chain Mouse mAb (Ab103488) at 1/3000 dilution
Samples: Lysates at 20 µL per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 52 kDa
Exposure time: 76.1 s

Western Blot: Fibrinogen beta chain Mouse mAb (Ab103488)

Fibrinogen beta chain Mouse mAb (Ab103488) - Western Blot
All lanes: Fibrinogen beta chain Mouse mAb (Ab103488) at 1/3000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 56 kDa
Observed band size: 47 kDa
Exposure time: 100.0 s

IHC: Fibrinogen beta chain Mouse mAb (Ab103488)

Fibrinogen beta chain Mouse mAb (Ab103488) - IHC
DAB staining on IHC
Samples: Human Kidney Tissue
Primary Ab: 10 µg/mL Fibrinogen beta chain Mouse mAb (Ab103488)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Fibrinogen beta chain Mouse mAb (Ab103488)

Fibrinogen beta chain Mouse mAb (Ab103488) - IHC
DAB staining on IHC
Samples: Human Liver Tissue
Primary Ab: 10 µg/mL Fibrinogen beta chain Mouse mAb (Ab103488)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: Fibrinogen beta chain Mouse mAb (Ab103488)

Fibrinogen beta chain Mouse mAb (Ab103488) - IHC
DAB staining on IHC
Samples: Human Placenta Tissue
Primary Ab: 10 µg/mL Fibrinogen beta chain Mouse mAb (Ab103488)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Anwendung

• Anwendung: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-2 µg/mL ;

Zugehörige Ziele (menschlich)

FGB Tbio Fibrinogen beta chain (0 Activities)

Discover Target: FGB

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Fibrinogen beta chain Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.