Fibrinogen gamma chain Mouse mAb - Primärer Antikörper, specific to Fibrinogen gamma chain, Mouse IgG1

Cat. No.: Ab103501

GRADE & PURITY Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

K24024_2E1

Conjugation

Unconjugated

Übersicht

Mouse anti Human Fibrinogen gamma chain Antibody, Monoclonal (K24024_2E1), could be used for WB, IHC, IP and so on.
Application:
WB: 1/1000
IHC: 1/500
IP: 1/100 
Protein Function：  
Fibrinogen has a double function: yielding monomers that polymerize into fibrin and acting as a cofactor in platelet aggregation.

Specifications

Product Name

Fibrinogen gamma chain Mouse mAb - Primärer Antikörper, specific to Fibrinogen gamma chain, Mouse IgG1

Synonyme

FGG antibody | FIBG_HUMAN antibody | Fibrinogen gamma chain antibody | Fibrinogen gamma polypeptide antibody | fibrinogen gamma-b chain antibody

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Validated, 1.0 mg/mL

Wirtsspezies

Maus

Spezifität

Fibrinogen gamma chain

Immunogen

Recombinant protein of human FGG (AA 61-160)

Positive Kontrolle

WB: HepG2 lysates. IHC: Human kidney tissue. IP: HepG2 lysates.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Fibrinogen gamma chain Mouse mAb (Ab103501)

Fibrinogen gamma chain Mouse mAb (Ab103501) - Western Blot
All lanes: Fibrinogen gamma chain Mouse mAb (Ab103501) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 52 kDa
Observed band size: 50 kDa
Exposure time: 30.0 s

IHC: Fibrinogen gamma chain Mouse mAb (Ab103501)

Fibrinogen gamma chain Mouse mAb (Ab103501) - IHC
IHC analysis of kidney tissue by Fibrinogen gamma chain Mouse mAb (Ab103501).IHC was performed using sections of the formalin-fixed paraffin-embedded kidney tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with Fibrinogen gamma chain Mouse mAb (Ab103501) at 2 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control.Result: Cells in glomeruli are positively stained at the cytoplasm.

IP: Fibrinogen gamma chain Mouse mAb (Ab103501)

Fibrinogen gamma chain Mouse mAb (Ab103501) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of Fibrinogen gamma chain Mouse mAb (Ab103501) with Hep G2 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. Fibrinogen gamma chain Mouse mAb (Ab103501) at 1 µg/mL was used as the primary antibody and peroxidase conjugated rabbit anti-mouse IgG (Light chain specific) was used as the secondary antibody. Lane 1: Hep G2 lysateLane 2: FGG immunoprecipitated from Hep G2 lysate by Fibrinogen gamma chain Mouse mAb (Ab103501)
Lane 3: The isotype control antibody instead of Fibrinogen gamma chain Mouse mAb (Ab103501) in Hep G2 lysate. Result: Fibrinogen gamma chain Mouse mAb (Ab103501) can immunoprecipitate FGG.

Anwendung

Anwendung

Dilution info

WB

1/1000

IP

1/100

IHC

1/500

Zugehörige Ziele (menschlich)

FGG Tbio Fibrinogen gamma chain (0 Activities)

Discover Target: FGG

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Fibrinogen gamma chain Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.