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GFAP Mouse mAb - Primary antibody, specific to GFAP, IgG2a

Cat. No.: Ab179925
COA
GFAP Mouse mAb - by Aladdin Scientific
AVAILABLE TO ORDER
GRADE & PURITY Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. Azide Free ? Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory. High Performance ? High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short. PBS Only ? PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers. See COA
Application
WB
Host species
Mouse
Reactive species
Human, Mouse
Isotype
Mouse IgG2a
Clone Number
EF02/4C5
Conjugation
Unconjugated
★
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab179925-10μl
≥10
$79.90
50μl
Ab179925-50μl
2
$259.90
100μl
Ab179925-100μl
2
$419.90
1ml
Ab179925-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
Bulk / Custom
♡ Wish List ⇅ Compare ⎙ Print page
SDS
Safety data
COA
Certificate
DS
Datasheet
SP
Spec sheet
Overview Specifications Storage and Shipping Images Application Documentation Advanced Data Associated Targets(Human) Certificates（CoA,COO,BSE/TSE and Analysis Chart) Documents & Articles Solution Calculators Reviews
🧪

Why this grade

Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,See COA Azide Free,Carrier Free,ExactAb™,High Performance,PBS Only,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Application
WB: 1/2,000-1/10,000 ; Predicted molecular weight: 50 kDa

Specifications

Product Name
GFAP Mouse mAb - Primary antibody, specific to GFAP, IgG2a
Synonyms
wu:fb34h11 antibody | ALXDRD antibody | cb345 antibody | etID36982.3 antibody | FLJ42474 antibody | FLJ45472 antibody | GFAP antibody | GFAP_HUMAN antibody | gfapl antibody | Glial fibrillary acidic protein antibody | Intermediate filament protein antibod
Specifications & Purity
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, See COA
Host species
Mouse
Specificity
GFAP
Immunogen
Recombinant full length protein corresponding to Human GFAP
Positive Control
WB: Mouse brain tissue lysate
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Isotype
IgG2a
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

GFAP Mouse mAb (Ab179925) - Western Blot
All lanes: Vinculin Antibody (Ab179925) at 0.5 μg/mL
Samples: Lysate at 20 µg
Secondary: Goat Anti-Mouse IgG H&L (HRP) at 1/20000 dilution

Predicted band size: 50.0 kDa
Observed band size: 46.0 kDa
Exposure time: 66.3 s 

Application
ApplicationDilution info
WB

1/2000-1/10000 ; Predicted molecular weight: 50 kDa

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
GFAP Tbio Glial fibrillary acidic protein (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

3 results found

Lot NumberCertificate TypeDateItem
ZJ23F1202279Certificate of AnalysisDec 28, 2023 Ab179925
ZJ23F1202268Certificate of AnalysisDec 28, 2023 Ab179925
ZJ23F1202269Certificate of AnalysisDec 28, 2023 Ab179925
Documents & Articles
In-cell ELISA protocol
Categories: Protocols
Tags:
Monoclonal antibodies In-cell ELISA Cytoblot Protein quantification HRP-Conjugated Secondary Antibodies Polyclonal Antibodies
Guidelines of Primary Antibodies for Western Blotting
Categories: Protocols
Tags:
Western blotting Monoclonal antibodies Recombinant Antibodies Polyclonal Antibodies Primary Antibodies Selection Loading Controls
Protocol of Western Blotting For High Molecular Weights
Categories: Protocols
Tags:
Western blotting Biological buffer Immunoblotting Protein Electrophoresis High Molecular Weights large molecular proteins WB antibody
Sodium Azide Removal Protocol
Categories: Protocols
Tags:
Recombinant antibody Sodium Azide Removal Antibody Purification Sephadex G-25 Sodium Azide-Free Antibody
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Carrier Free grade guide → View Validated grade guide → View ExactAb™ grade guide → View Azide Free grade guide → View High Performance grade guide → View PBS Only grade guide →
Bulk & Custom Packaging Need larger quantities or custom specifications? Contact our team for a tailored quote.

View related series

Click to view target information: GFAP
 Accession#:P14136 (7) Gene ID:2670 (6) GFAP (6) Loading control (34)

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