GlcNAc-1-P uridyltransferase (PmGlmU)

Cat. No.: G1445241

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥85%(SDS-PAGE), ≥5 U/mg protein; Protein concentration: See COA; expressed in E.coli

Accession #

Q9CK29

Protein Tag

C-His

Expression system

E. coli

Bioactivity

≥5 U/mg protein

Overview

N-Acetylglucosamine 1-phosphate uridyltransferase (PmGlmU), EC 2.3.1.157, derived from Pasteurella multocida. This enzyme is used to catalyze the formation of UDP‑GlcNAc from GlcNAc‑1‑P, working in conjunction with PmPPA and NahK to activate GlcNAc. The product is recombinantly expressed in E. coli and exhibits high activity.

Reaction

GlcNAc‑1‑P + UTP → UDP‑GlcNAc + PPi

Protocol

Add substrates to the reaction system according to the desired composition. Include 5 mM MgCl₂ and, if desired, a buffer salt such as Tris‑HCl. Adjust the pH to 7.5–8.5, then add an appropriate amount of PmGlmU (depending on the reaction requirements).

Mix thoroughly and incubate the sample at 37 °C for 30–60 min. The recommended reaction buffer is Tris‑HCl (pH 8.0), but other common biological buffers such as Tris or PBS are also suitable.

Note: When scaling up the system, the incubation time or enzyme amount should be adjusted according to actual conditions; low‑speed shaking may be used to ensure proper mixing.

Precautions

For your safety and health, please wear a lab coat and disposable gloves when handling this product. This product is for research use only!

Specifications

Product Name

GlcNAc-1-P uridyltransferase (PmGlmU)

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥85%(SDS-PAGE), ≥5 U/mg protein; Protein concentration: See COA; expressed in E.coli

Bioactivity

≥5 U/mg protein

Accession #

Q9CK29

Predicted molecular weight

57 kDa

Enzyme Commission Number

EC 2.3.1.157

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥5 U/mg protein; Protein concentration: See COA; expressed in E.coli

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

The amount of enzyme required to catalyze the formation of 1 μmol UDP-GlcNAc from GlcNAc-1-P and UTP per minute at 37°C