β-Glucuronidase from Helix pomatia, CAS No.9001-45-0

CAS: 9001-45-0 Cat. No.: G755255 EC Number: 232-606-8

GRADE & PURITY Type HP-2, aqueous solution,≥100,000 units/mL

Accession #

P09811

Overview

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis

Application:

• Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
• Removal of glucuronide conjugates such as for the preparation of Baicalein.-

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

Specifications

Product Name

β-Glucuronidase from Helix pomatia, CAS No.9001-45-0

Specifications & Purity

Type HP-2, aqueous solution, ≥100, 000 units/mL

Biochemical and Physiological Mechanisms

Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and

Accession #

P09811

CAS

9001-45-0

Enzyme Commission Number

3.2.1.31(BRENDA, IUBMB)

Molecule Type

Enzyme

Storage and Shipping

Concentration

Type HP-2, aqueous solution,≥100,000 units/mL

Storage

Store at 2-8°C

Shipped In

Wet ice

References

1. Bairen Du, Jingjing Zhang, Yanjie Dong, Junwei Wang, Longwen Lei, Rengfei Shi.  (2020)  Determination of testosterone/epitestosterone concentration ratio in human urine by capillary electrophoresis.  STEROIDS,      [PMID:32603755] [10.1016/j.steroids.2020.108691]

2. Di Wu, Na Hu, Jichao Liu, Guangsen Fan, Xiuting Li, Jing Sun, Chunji Dai, Yourui Suo, Guoliang Li, Yongning Wu.  (2018)  Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection.  TALANTA,      [PMID:30172485] [10.1016/j.talanta.2018.07.073]