Goat Anti-Human IgG Fc (FITC) - Secondary antibody, specific to Human IgG Fc

Cat. No.: Ab216758
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.0 mg/mL
Application
Flow Cytometry, IF/ICC
Host species
Goat
Reactive species
Human
Isotype
Goat IgG
Conjugation
FITC
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μg
Ab216758-10μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$19.90
100μg
Ab216758-100μg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$59.90
1mg
Ab216758-1mg
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$199.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 1.0 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Protected from light,Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Goat Anti-Human IgG Fc Antibody, Secondary antibody, could be used for Flow Cytometry, IF and so on.

Specifications

Product Name
Goat Anti-Human IgG Fc (FITC) - Secondary antibody, specific to Human IgG Fc
Synonyms
Goat Anti-Human IgG Fc Antibody | Goat Anti Human IgG Fc Antibody | Anti-Human IgG Fc Antibody | Anti Human IgG Fc Antibody
Specifications & Purity
ExactAb™, Validated, 1.0 mg/mL
Host species
Goat
Specificity
Human IgG Fc
Immunogen
Human IgG Fc
Conjugation
FITC
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
Grade
ExactAb™, Validated
Purification Method
antigen affinity chromatography
Concentration
1.0 mg/mL
Storage and Shipping
Storage
Protected from light,Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20°C. Avoid freeze/thaw cycle. Protect from light.
Images

Goat Anti-Human IgG Fc (FITC) (Ab216758) - IF
IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Citatuzumab (anti-EpCAM) (Ab209902) at 1/1000 dilution in blocking buffer overnight at 4°C, and then incubated with Goat Anti-Human IgG Fc (FITC) (Ab216758) at a dilution of 1/1000 for 1 hour at room temperature in the dark (green). PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.

Goat Anti-Human IgG Fc (FITC) (Ab216758) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD20 (red) with Ibritumomab (anti-CD20) (Ab191926). Goat Anti-Human IgG Fc (FITC) (Ab216758) at a dilution of 1/300 was used as the secondary antibody. Blue - Isotype control, Human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Application
ApplicationDilution info
Flow Cytometry

1/100-1/1000

IF/ICC

1/100-1/1000

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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