GRADE & PURITYValidated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Pre-adsorbed?Pre-adsorbed — cross-reactive antibodies removed by adsorption for higher specificity. Use in multiplex/IHC to cut background and cross-reaction.See COA
ExactAb™, Validated, Pre-adsorbed, See COA ExactAb™,Pre-adsorbed,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at 2-8°C,Protected from light,Do not freeze Ships Wet ice,Do not freeze Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Specifications
Product Name
Goat Anti-Rabbit IgG H&L (FITC) (Ready to use) - Secondary antibody, specific to Rabbit IgG
Fluorescent secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from rabbit. Ready-to-use secondary antibody concentrations have been optimized and tested by immunofluorescent staining with Flow Cytometry and IF/
Host species
Goat
Specificity
Rabbit IgG
Immunogen
Rabbit IgG
Conjugation
FITC
Excitation (nm)
Blue Laser(488nm)
Emission(nm)
517nm
Ex/Em(nm)
Ex:498nm;Em:517nm
Grade
ExactAb™, Pre-adsorbed, Validated
Concentration
See COA
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
This product can be stored at 2-8°C for 12 months. Do not freeze. Store in the dark.
Images
Goat Anti-Rabbit IgG H&L (FITC) (Ready to use) (Ab226631) - IF/ICC IF analysis of EpCAM (green) in HT-29 cells. The cells were fixed with 100% methanol for 5 minutes, and blocked with 5% BSA for 1 hour at room temperature. Cells were stained with Recombinant EpCAM Antibody (Ab101839) at 1/1000 dilution in blocking buffer overnight at 4°C. Then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ready to use) (Ab226631) at 2mL/well for 1 hour at room temperature in the dark (green). A 6-well cell culture plate was used for this experiment. PBS instead of the primary antibody was used as the secondary antibody only control. Cells were counterstained with DAPI (blue). Images were taken on the confocal laser scanning microscope.
Goat Anti-Rabbit IgG H&L (FITC) (Ready to use) (Ab226631) - Flow Cytometry Flow Cytometry analysis of HA tag expression by HEK293T cells transfected with a mCherry-HA tag (red) compared to an isotype control (blue). The cells were fixed with 100% methanol for 5 minutes and then stained with Recombinant HA tag Antibody (Ab106771) at 0.5 μg/mL for 30 minutes at 4°C in the dark. Then incubated with Goat Anti-Rabbit IgG H&L (FITC) (Ready to use) (Ab226631) at 100 μL/well was used as the secondary antibody. Blue - Rabbit IgG (Ab170215).
Application
Application
Dilution info
IF/ICC
Use at an assay dependent volume.
Flow Cytometry
100μL/test
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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