GRB2 Mouse mAb - Primärer Antikörper, specific to GRB2, Mouse IgG1

Cat. No.: Ab106387

GRADE & PURITY Validated, ExactAb™, 4.2 mg/mL

Application

WB

Host species

Maus

Reactive species

Maus, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

5G3-E7-F11

Conjugation

Unconjugated

Übersicht

Mouse anti Human GRB2 Antibody, Monoclonal (5G3-E7-F11), could be used for WB and so on .
Application:
WB: 1/500-1/1000
Protein Function
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.
Isoform GRB3-3 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform GRB3-3 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death.

Specifications

Product Name

GRB2 Mouse mAb - Primärer Antikörper, specific to GRB2, Mouse IgG1

Synonyme

GRB2 | ASH | Growth factor receptor-bound protein 2 | Adapter protein GRB2 | Protein Ash | SH2/SH3 adapter GRB2 | Abundant SRC homology antibody | Adapter protein GRB2 antibody | ASH antibody | Ash protein antibody | EGFRBP GRB2 antibody | Epidermal growt

Spezifikationen & Reinheit

ExactAb™, Validated, 4.2 mg/mL

Wirtsspezies

Maus

Spezifität

GRB2

Immunogen

Recombinant protein of human GRB2 (AA 1-217).

Positive Kontrolle

WB：K562, Raji, Jurkat, Ramos, C6, NIH/3T3, HeLa, MCF7 and A431 cell lysates.

Konjugation

Unconjugated

Note

ExactAb™, Validated

Isotyp

Mouse IgG1

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

4.2 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: GRB2 Mouse mAb (Ab106387)

GRB2 Mouse mAb (Ab106387) - Western Blot
All lanes: GRB2 Mouse mAb (Ab106387) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040)at 1/20000 dilution

Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 7.2 s

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/1000

Zugehörige Ziele (menschlich)

GRB2 Tchem Growth factor receptor-bound protein 2 (0 Activities)

Discover Target: GRB2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GRB2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.