GRP78 BiP Mouse mAb - Primärer Antikörper, specific to GRP78 BiP, Mouse IgG2b

Cat. No.: Ab106528

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), See COA

Application

Animal Model, Blocking, Flow Cytometry, Functional Assay, WB

Host species

Maus

Reactive species

Maus, Mensch

Isotype

Mouse IgG2b

Clone Number

C38

Conjugation

Unconjugated

Übersicht

Mouse anti Human/Mouse GRP78 BiP Antibody, Monoclonal (C38), could be used for in vivo GRP78 blockade, in vitro GRP78 blockade, WB, Flow and so on.

Application:

in vivo GRP78 blockade: Use at an assay dependent concentration. 

in vitro GRP78 blockade: Use at an assay dependent concentration. 

WB: Use at an assay dependent concentration.

Flow: Use at an assay dependent concentration.
Protein Function
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.

Specifications

Product Name

GRP78 BiP Mouse mAb - Primärer Antikörper, specific to GRP78 BiP, Mouse IgG2b

Synonyme

HEL-S-89n | Bip | Sez7 | mBiP | Grp78 | SEZ-7 | Hsce70 | baffled | D2Wsu17e | D2Wsu141e | 78 kDa glucose-regulated protein | BIP | Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 | FLJ26106 | GRP78 | GRP-78 | GRP78BIP | Heat shock 70 kDa protei

Spezifikationen & Reinheit

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),See COA

Wirtsspezies

Maus

Spezifität

GRP78 BiP

Immunogen

Recombinant protein of murine GRP78 (AA 1-655)

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Isotyp

Mouse IgG2b

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at 2-8°C,Do not freeze

Verschickt in

Wet ice,Do not freeze

Stabilität und Lagerung

Shipped at 4°C. Store at 4°C long term (12 months). Do not freeze. Upon receipt, it is recommended to aliquot.

Bilder

Flow Cytometry: GRP78 BiP Mouse mAb (Ab106528)

GRP78 BiP Mouse mAb (Ab106528) - Flow Cytometry
Flow Cytometry analysis of A431 cells labelling GRP78 BiP (red) with GRP78 BiP Mouse mAb (Ab106528) at 0.1 μg/mL for 1 hour at 4°C. Goat Anti-Mouse IgG (PE) (Ab179006) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, mouse IgG (Ab170221). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

• Anwendung: Dilution info
• WB:

  Use at an assay dependent concentration.;

• Flow Cytometry:

  Use at an assay dependent concentration.

• in vivo GRP78 blockade:

  Use at an assay dependent concentration ;

• in vitro GRP78 blockade:

  Use at an assay dependent concentration ;

Zugehörige Ziele (menschlich)

HSPA5 Tchem Endoplasmic reticulum chaperone BiP (0 Activities)

Discover Target: HSPA5

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at 2–8 °C. Do not freeze. Freezing may cause irreversible damage to this material.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (GRP78 BiP Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.