Hemicellulase from Aspergillus niger, CAS No.9025-56-3

CAS: 9025-56-3 Cat. No.: H304918 EC Number: 232-799-9

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥50 U/mg enzyme powder

Bioactivity

≥50 U/mg enzyme powder

Overview

Hemicellulase is produced by refined submerged fermentation using a high-yield strain of Aspergillus niger obtained through mutagenesis and screening. Hemicellulose, a major component of plant cell walls, is a heterogeneous polymer composed of several types of monosaccharides, including pentoses and hexoses such as xylan, mannan, and galactan.

The main component of this hemicellulase product is mannanase, which functions to degrade hemicellulose and eliminate anti-nutritional factors. This enzyme can be widely used in food, feed, textile, paper, petroleum, and other industries.

Product Characteristics

The applicable pH range for hemicellulase is 3.0–6.5, with an optimum pH of 3.5–5.0. The temperature range is 30–70°C, with an optimum temperature of 40–55°C.

Enzyme Activity (β-mannanase Activity)

One unit (U) of β-mannanase activity is defined as the amount of enzyme required to release 1 μmol of reducing sugar per minute from a 3 mg/mL mannan solution at 37°C and pH 5.5.

Usage Method

The recommended dosage is 0.01–1.0 kg per ton of raw material. The specific addition method and dosage should be determined through experiments.

Safety Precautions

Enzyme preparations are proteins. Inhalation of dust or aerosols may cause allergic reactions. Prolonged contact with certain enzymes may irritate the skin, eyes, and mucous membranes; splashing or vigorous agitation may generate respirable dust. It is recommended to wear protective clothing, gloves, and eye or face protection.

Storage and Handling Notes

This product is an active biological preparation. During transportation and storage, it should be kept away from light, at low temperature, in a dry and well-ventilated environment.

Specifications

Product Name

Hemicellulase from Aspergillus niger, CAS No.9025-56-3

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥50 U/mg enzyme powder

Biochemical and Physiological Mechanisms

Hemicellulase can randomly hydrolyze mannan and other components to degrade hemicellulose, with the main products being manno-oligosaccharides and a small amount of mannose.

Bioactivity

≥50 U/mg enzyme powder

CAS

9025-56-3

Enzyme Commission Number

EC 3.2.1.78

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥50 U/mg enzyme powder

Storage

Store at 2-8°C,Protected from light,Desiccated

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (12 months). Store in the dark. Desiccated.

Unit definition

One unit (U) of mannanase activity is defined as the amount of enzyme required to release 1 μmol of reducing sugar per minute from a 3 mg/mL mannan solution at 37°C and pH 5.5.

References

1. Jing Wang, Yumin Song, Futong Cui, Wenbin Wu, Haodong Zhu, Xiaoping Li, Kunyong Kang.  (2023)  Preparation and characterization of nanocellulose fiber (CNF) by biological enzymatic method.  JOURNAL OF THERMOPLASTIC COMPOSITE MATERIALS,      [PMID:] [10.1177/08927057231180479]

2. Xushuo Yuan, Wentao Yao, Decai Ji, Li Liu, Yanfei Lin, Heyang Zeng, Tao Jin, Kaimeng Xu, Guanben Du, Lianpeng Zhang.  (2023)  Synthesis of corn bract cellulose-based Au3+ fluorescent probe and its application in composite membranes.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:37105254] [10.1016/j.ijbiomac.2023.124600]

3. Xushuo Yuan, Jiaxin Zhao, Xiaoxiao Wu, Wentao Yao, Haiyang Guo, Decai Ji, Qingkai Yu, Liwen Luo, Xiaoping Li, Lianpeng Zhang.  (2022)  Extraction of Corn Bract Cellulose by the Ammonia-Coordinated Bio-Enzymatic Method.  Polymers,  15  (1): (206).  [PMID:36616555] [10.3390/polym15010206]

4. Jiaxin Zhao, Xiaoxiao Wu, Xushuo Yuan, Xinjie Yang, Haiyang Guo, Wentao Yao, Decai Ji, Xiaoping Li, Lianpeng Zhang.  (2022)  Nanocellulose and Cellulose Making with Bio-Enzymes from Different Particle Sizes of Neosinocalamus Affinis.  Coatings,  12  (11): (1734).  [PMID:] [10.3390/coatings12111734]

5. Xiaobao Li, Zhengjie Tang, Zhenbing Sun, John Simonsen, Zhinan Luo, Xiaoping Li, Jeffery J. Morrell.  (2022)  Chemical and Enzymatic Fiber Modification to Enhance the Mechanical Properties of CMC Composite Films.  Polymers,  14  (19): (4127).  [PMID:36236075] [10.3390/polym14194127]

6. Jiaojiao Yuan, Su Tuo, Xiuhong Shi, Junling Tu.  (2024)  A new combined technology: Macroporous adsorption resin and high speed counter current chromatography for hydroxytyrosol separation from olive leaf enzymatic hydrolysate.  JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,      [PMID:38394917] [10.1016/j.jchromb.2024.124058]