HERPUD1 Mouse mAb - Primärer Antikörper, specific to HERPUD1

Cat. No.: Ab107327

GRADE & PURITY Validated, ExactAb™, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Maus

Reactive species

Maus, Mensch, Rat

Isotype

Mouse IgG2a

Clone Number

OTI1A2

Conjugation

Unconjugated

Übersicht

Mouse anti Human HERPUD1 Antibody, Monoclonal (OTI1A2), could be used for WB, IHC, IF/ICC and so on.

Specifications

Product Name

HERPUD1 Mouse mAb - Primärer Antikörper, specific to HERPUD1

Synonyme

SUP | HERP | Mif1 | HERPUD1 | HERP antibody | HERP1_HUMAN antibody | HERPUD1 antibody | Homocysteine inducible endoplasmic reticulum stress inducible ubiquitin like domain member 1 antibody | Homocysteine inducible endoplasmic reticulum stress inducible u

Spezifikationen & Reinheit

ExactAb™, Validated, High Performance, See COA

Biochemische und physiologische Mechanismen

Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Could enhance presenilin-mediated beta-amyloid prote

Wirtsspezies

Maus

Spezifität

HERPUD1

Immunogen

Recombinant full length protein of human HERPUD1 produced in HEK293T.

Positive Kontrolle

WB: Hep G2, HeLa, A549, Jurkat, MCF7, NIH/3T3 and C6 cell lysates. IHC: Human kidney, pancreas, pancreas carcinoma, endometrium and prostate tissues. IF/ICC: COS7 cells transfected with the pCMV6-ENTRY HERPUD1.

Konjugation

Unconjugated

Note

ExactAb™, High Performance, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - Western Blot
All lanes: HERPUD1 Mouse mAb (Ab107327) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 51 kDa
Exposure time: 80.0 s

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IHC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IHC
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using HERPUD1 Mouse mAb (Ab107327). (Heat-induced epitope retrieval by 1 mM EDTA in 10 mM Tris, pH 8.5, 120 °C for 3 min, Ab107327)

IF/ICC: HERPUD1 Mouse mAb (Ab107327)

HERPUD1 Mouse mAb (Ab107327) - IF/ICC
HERPUD1 Mouse mAb (Ab107327) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HERPUD1.

Anwendung

• Anwendung: Dilution info
• IF/ICC:

  1/100;

• IHC:

  1/150

• WB:

  1/400-1/4000

Zugehörige Ziele (menschlich)

HERPUD1 Tbio Homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein (0 Activities)

Discover Target: HERPUD1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HERPUD1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.