His tag (C-terminal) Mouse mAb - Primary antibody, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
His tag (C-terminal) Mouse mAb - Primary antibody, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.PBS Only?PBS-only formulation — supplied in phosphate-buffered saline with no other additives. Use when you need a clean PBS buffer free of stabilizers/carriers.≥95%(SDS-PAGE)1.0 mg/mL
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti His tag (C-terminal) Antibody, clone IJ04/4D2, could be used for WB, ELISA and so on.
Specifications
Product Name
His tag (C-terminal) Mouse mAb - Primary antibody, specific to Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
Synonyms
6 His epitope tag antibody | Hexa His tag antibody | HHHHHH epitope tag antibody | HHHHHH tag antibody | His tag antibody | Polyhistidine Tag antibody | His tag Mouse Monoclonal Antibody | His tag Mouse mAb | Anti-His tag | Monoclonal Anti- His tag antibo
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized met
Host species
Mouse
Specificity
Recombinant proteins containing the 6X His tag® fused to carboxy terminus of fusion proteins.
Immunogen
A recombinant protein containing the sequence HHHHHH
Positive Control
WB: His-transfected HEK293 whole cell lysates; C-terminal His fusion proteins
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated
Light Chain Type
kappa
SDS-PAGE
150.0 KDa
Purification Method
Protein A purified
Concentration
1.0 mg/mL
Note
This product can replace Ab086831, Ab155835, Ab219147 and Ab219145.
Storage and Shipping
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 2-8℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
His tag (C-terminal) Mouse mAb (Ab219141) - Western Blot All lanes: His tag (C-terminal) Mouse Antibody (Ab219141) at 1/10000 dilution Samples: Lysates at 10 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 48,116 kDa Observed band size: 51,116 kDa Exposure time: 65.1 s
His tag (C-terminal) Mouse mAb (Ab219141) - Western Blot All lanes: His tag (C-terminal) Mouse Antibody (Ab219141) at 1/10000 dilution Samples: Lysates at 10 ng per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution Predicted band size: 35,70 kDa Observed band size: 35,67 kDa Exposure time: 60.0 s
Application
Application
Dilution info
WB
1/1000 - 1/20000
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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