HLA Class I Mouse mAb - Primärer Antikörper, specific to HLA Class I, Mouse IgG2a

Cat. No.: Ab108167

GRADE & PURITY Carrier Free, Low Endotoxin, Azide Free, Validated, PBS Only, ≥95%(SDS-PAGE&HPLC), See COA

Application

Flow Cytometry, Functional Assay, IF/ICC, IHC, IP, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG2a

Clone Number

W6/32

Conjugation

Unconjugated

Übersicht

Mouse anti Human HLA Class I Antibody, Monoclonal (W6/32), could be used for WB, Flow, IP, IHC, ICC/IF, Functional Assays and so on.
Application
ICC/IF: 5.0µg/mL
WB: 5.0µg/mL
Flow: 1.0-5.0µg/mL
IP: Use at an assay dependent concentration.
IHC: Use at an assay dependent concentration.
Functional Assays: Use at an assay dependent concentration.

Specifications

Product Name

HLA Class I Mouse mAb - Primärer Antikörper, specific to HLA Class I, Mouse IgG2a

Synonyme

A-10 alpha chain | FLJ26655 | HLA class I histocompatibility antigen, A-1 alpha chain | HLA class I histocompatibility antigen, A-28 alpha chain | HLA class I histocompatibility antigen, A-9 alpha chain | HLA Class I | HLAA | HLA-A | HLA-ABC | major histo

Spezifikationen & Reinheit

Carrier Free,Low Endotoxin,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE&HPLC),See COA

Wirtsspezies

Maus

Spezifität

HLA Class I

Immunogen

Human tonsil cell membrane

Positive Kontrolle

Flow Cyt: Jurkat cells. ICC: HeLa cells.

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, Low Endotoxin, PBS Only, Validated

Isotyp

Mouse IgG2a

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Product Line

Biologische Produkte

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Flow Cytometry: HLA Class I Mouse mAb (Ab108167)

HLA Class I Mouse mAb (Ab108167) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling HLA Class I with HLA Class I Mouse mAb (Ab108167) conjugated with biotin (right panel) at 0.5 μg/mL for 1 hour at 4°C, compared with Mouse IgG Isotype Control Antibody (Biotin) (Ab180306) (left panel) and detected with SA-PE (rp156150). 

Flow Cytometry: HLA Class I Mouse mAb (Ab108167)

Flow Cytometry analysis of human peripheral blood lymphocytes labelling HLA Class I (red) with HLA Class I Mouse mAb (Ab108167) conjugated with biotin at 0.5 μg/mL for 1 hour at 4°C, and detected with SA-PE (rp156150). Blue - Mouse IgG Isotype Control Antibody (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.

Anwendung

Anwendung

Dilution info

IF/ICC

5.0 μg/mL

Functional Assay

Use at an assay dependent concentration.

IP

Use at an assay dependent concentration.

Flow

1-5 μg/mL

IHC

Use at an assay dependent concentration.

WB

5.0µg/mL

Zugehörige Ziele (menschlich)

HLA-A Tbio HLA class I histocompatibility antigen, A-69 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-74 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-26 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-24 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-68 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-11 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-80 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-43 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-34 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-2 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-66 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-23 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-32 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-33 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-29 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-30 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-36 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tclin HLA class I histocompatibility antigen, A-3 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-1 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-25 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

HLA-A Tbio HLA class I histocompatibility antigen, A-31 alpha chain (0 Activities)

Discover Target: HLA-A

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does Low Endotoxin mean?

Low Endotoxin indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (HLA Class I Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.