Hsp27 Mouse mAb - Primärer Antikörper, specific to Hsp27

Cat. No.: Ab156228

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG2b

Clone Number

CD05/2A2

Conjugation

Unconjugated

Übersicht

Mouse anti Human Hsp27 Antibody, clone CD05/2A2, could be used for WB, Flow Cytometry, IF/ICC, ELISA and so on.

Specifications

Product Name

Hsp27 Mouse mAb - Primärer Antikörper, specific to Hsp27

Synonyme

Heat shock 27kDa protein antibody | 28 kDa heat shock protein antibody | CMT2F antibody | DKFZp586P1322 antibody | epididymis secretory protein Li 102 antibody | Estrogen regulated 24 kDa protein antibody | Estrogen-regulated 24 kDa protein antibody | Hea

Spezifikationen & Reinheit

Carrier Free,ExactAb™,Azide Free,Validated,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Biochemische und physiologische Mechanismen

Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numer

Wirtsspezies

Maus

Spezifität

Hsp27

Immunogen

Recombinant protein corresponding to Human Hsp27

Positive Kontrolle

WB: MCF-7, HeLa, K562, A431, A549 and LNCaP whole cell lysates Flow Cytometry: HeLa cells IF/ICC: HeLa cells

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, PBS Only, Validated

Lichterketten-Typ

kappa

SDS-PAGE

150.0 KDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Lagerung und Versand

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Bilder

Western Blot: Hsp27 Mouse mAb (Ab156228)

Hsp27 Mouse mAb (Ab156228) - Western Blot
All lanes: Hsp27 Mouse Antibody (Ab156228) at 1/20000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 23 kDa
Observed band size: 27 kDa
Exposure time: 7.6 s 

Flow Cytometry: Hsp27 Mouse mAb (Ab156228)

Hsp27 Mouse mAb (Ab156228) - Flow Cytometry 
Flow Cytometry analysis of HeLa cells stained with Hsp27 Mouse mAb (Ab156228) (Red) at 0.625 μg/mL for 1 hour at 4°C. Cells were fixed and permeabilized with 100% methanol. The Goat Anti-Mouse IgG H&L (PE) (Ab179006) was used as the secondary antibody. Unlabeled sample (Black) was used as a negative control. The mouse IgG2b was used as an isotype control (Blue).​

ICC/IF: Hsp27 Mouse mAb (Ab156228)

Hsp27 Mouse mAb (Ab156228) - ICC/IF
IF analysis of Hsp27 (Green) in HeLa cells. The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. Cells were stained with Hsp27 Mouse mAb (Ab156228) at 5.0 μg/mL in blocking buffer for 2 hour at RT and then incubated with Goat Anti-Mouse IgG H&L (FITC) (Ab179003) at a dilution of 1/1000 for 1 hour at room temperature in the dark. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Anwendung

• Anwendung: Dilution info
• IF/ICC:

  1/200;

• WB:

  1/1000 - 1/20000 ;

• Flow Cytometry:

  1/200 - 1/50000;

Zugehörige Ziele (menschlich)

HSPB1 Tchem Heat shock protein beta-1 (0 Activities)

Discover Target: HSPB1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Hsp27 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.