Human Adiponectin ELISA Kit - Bioactive, for ELISA, for enzyme immunoassay(ELISA)

Cat. No.: H981627

GRADE & PURITY Bioactive, for Enzyme immunoassay(ELISA), for ELISA

Synonyms

Human Adiponectin Enzyme-Linked ImmunoSorbent Assay Kit | Adiponectin ELISA Kit | 30 kDa adipocyte complement related protein | 30 kDa adipocyte complement-related protein | ACDC | Acrp 30 | ACRP30 | ADIPO_HUMAN | Adipocyte | Adipocyte C1q and collagen do

Storage

Store at 2-8°C

Shipped In

Wet ice

Application

ELISA

Specifications

Synonyme

Human Adiponectin Enzyme-Linked ImmunoSorbent Assay Kit | Adiponectin ELISA Kit | 30 kDa adipocyte complement related protein | 30 kDa adipocyte complement-related protein | ACDC | Acrp 30 | ACRP30 | ADIPO_HUMAN | Adipocyte | Adipocyte C1q and collagen do

Spezifikationen & Reinheit

Bioactive, for ELISA, for enzyme immunoassay(ELISA)

Stabilität und Lagerung

Store at 2-8℃ (6 months).

Storage

Store at 2-8°C

Verschickt in

Wet ice

Dieses Produkt erfordert Kühlkettenversand. Grundversand und andere Economy-Optionen sind nicht verfügbar.

Note

Bioactive, for ELISA, für den Enzymimmunoassay (ELISA)

Product Line

Biologische Produkte

Zugehörige Ziele (menschlich)

ADIPOQ Tbio Adiponectin (0 Activities)

Discover Target: ADIPOQ

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Application Protocols

Only protocol details explicitly provided by the manufacturer should be followed.

• Item-specific facts (from Product Data)
  • Manufacturer Applications (verbatim): Not specified for this item; refer to the kit insert.
  • Recommended sample types, dilutions, incubation times, wash steps, substrate/stop, readout wavelength: Not specified for this item; refer to the kit insert.

Please consult the Instruction Manual/CoA included with the kit for:

• Plate layout and standard curve preparation
• Sample handling requirements and acceptable matrices
• Step-by-step workflow with incubation times/temperatures and wash cycles
• Calculation method (standard curve fitting, dilution corrections)
• Acceptance criteria and troubleshooting

Note: Do not substitute reagents or alter steps without guidance from the official protocol.

Biological Roles

Adiponectin (also known as ACRP30, AdipoQ) is a secreted adipokine predominantly produced by adipocytes.

• General biological functions (literature; not kit-specific)
  • Modulates glucose and lipid metabolism, influencing insulin sensitivity in peripheral tissues.
  • Activates signaling via adiponectin receptors (AdipoR1/AdipoR2), engaging AMPK and PPAR-related pathways associated with energy homeostasis and fatty acid oxidation.
  • Circulates in multiple oligomeric forms (low-, medium-, and high-molecular-weight complexes) with distinct bioactivities and tissue distributions.
  • Implicated in inflammatory tone regulation within metabolic tissues and the vasculature in preclinical models.
• Experimental considerations (general)
  • Circulating levels can be affected by physiological variables (nutritional status, sex, BMI) and sample handling (coagulation, hemolysis, storage cycles).
  • Assay design may target total adiponectin or specific oligomeric states; verify what this kit measures in the Instruction Manual.
• Research applications (general)
  • Quantification in serum/plasma for metabolic research, adipocyte biology, and cell culture supernatants from adipocyte or hepatocyte models.

Note: This section summarizes biochemical roles for research context only. No medical or clinical claims are made. Always consult primary literature for mechanistic details relevant to your study.

Buffer Applications

The Human Adiponectin ELISA Kit includes or specifies buffers tailored to immunoassay performance; do not substitute unless instructed.

• Item-specific facts (from Product Data)
  • Specific buffer compositions and pH: Not specified for this item; refer to the kit insert.
• General ELISA buffer guidance (not item-specific)
  • Coating/capture: Carbonate-bicarbonate buffer (~pH 9.4) or proprietary formulations promote antibody adsorption (for custom plates). Pre-coated plates in commercial kits typically require no coating step.
  • Blocking: Protein-based blockers (BSA, casein, fish gelatin) or polymeric blockers reduce nonspecific binding; salt and detergent (e.g., Tween 20) help minimize background.
  • Sample/standard diluent: Buffered protein matrix with detergent and carrier protein to stabilize analyte and reduce matrix effects.
  • Wash buffer: PBS or TBS with 0.05–0.2% Tween 20 is common; adequate wash cycles are critical for low background.
  • Substrate buffer: Optimized for the enzyme used (e.g., HRP/TMB systems), often light-sensitive; stop solution is typically dilute mineral acid.
• Practical tips
  • Match ionic strength and detergent levels across diluent and wash to maintain consistent stringency.
  • Filter or degas wash buffer to prevent bubble artifacts that interfere with OD readings.

Follow the specific buffer recipes, volumes per well, and preparation instructions in this kit’s manual.

Green Alternatives

While an ELISA kit is not a solvent or synthesis reagent, greener lab practices can reduce environmental impact during its use.

• Waste minimization
  • Prepare only the volume of wash buffer and reagents needed for the planned plate area (e.g., partial plate runs) to avoid surplus disposal.
  • Use reservoir inserts and low-dead-volume tips to reduce plastic waste and reagent loss.
• Safer chemicals and substitutions (general)
  • If the kit permits HRP/TMB chemistry, avoid more hazardous alternatives (e.g., OPD) and follow recommended neutralization for acidic stop solutions to meet local disposal requirements.
• Energy and resource efficiency
  • Consolidate runs to minimize repeated equipment warm-up and reduce cold storage door-open time.
  • Store at 2–8°C as specified; do not freeze components that are not meant to be frozen—this preserves function and reduces waste from failed runs.
• Comparative considerations (general)
  • Pre-coated, ready-to-use plate formats typically reduce preparation time and chemical handling compared with custom coating workflows, lowering overall solvent/chemical use and variability.

Always follow your institution’s green lab and hazardous waste disposal guidelines, and consult the SDS/kit insert for component-specific disposal instructions.

Pharmaceutical Uses

Not applicable to this product as a research-use-only immunoassay kit.

• Context (general)
  • ELISA kits like this are intended for non-clinical laboratory research use and method development. They are not cleared for diagnostic, therapeutic, or GMP release testing unless explicitly stated by the manufacturer and supported by appropriate validation and regulatory documentation.
  • If method translation to regulated environments is required, users must perform their own qualification/validation (e.g., accuracy, precision, specificity, linearity, range, robustness) under applicable guidelines.

No pharmacopeial status or excipient/formulation role applies to this item.

Physical Properties

This is a multi-component ELISA kit; bulk thermophysical properties of a single compound do not apply.

• Item-specific facts (from Product Data)
  • Appearance: Not specified for this item; refer to CoA/Spec Sheet.
  • Molecular Weight (of kit): Not applicable.
• Literature/general notes (for typical ELISA kit components; not item-specific)
  • Common components include buffered aqueous solutions (near-neutral pH), protein standards, antibody-coated plates or strips, enzyme conjugate, chromogenic substrate (e.g., TMB), stop solution (often acidic), and wash buffers.

Because individual reagent compositions and concentrations vary by lot and kit design, do not infer physical properties (density, refractive index, pH, melting/boiling points) without the kit insert/SDS for each component.

Actionable guidance

• Inspect each vial/solution upon receipt for clarity, absence of precipitate (unless lyophilized by design), and intact seals.
• For lyophilized components, reconstitution volume and solvent are kit-specific; see the insert.
• Temperature sensitivity: Store all components at 2–8°C per Product Data and avoid repeated temperature cycling. Minimize light exposure for chromogenic substrates.

Quality and Grades

• Item-specific facts (from Product Data)
  • Grade/Purity: Bioactive; for Enzyme immunoassay (ELISA); for ELISA.
  • Research use only.
• What this means (general context, not a specification)
  • Bioactive: Components (e.g., antibodies, standards) are suitable for biological binding/recognition in immunoassays, supporting specific detection of target antigen.
  • “For ELISA”: Reagents are formulated/qualified for ELISA workflows (e.g., appropriate buffers, stabilizers, and conjugates) to yield signal-to-noise and dynamic range consistent with immunoenzymatic detection.
• Quality considerations for ELISA kits (general guidance)
  • Lot-to-lot verification typically includes: standard curve performance, precision (intra-/inter-assay CV), specificity (minimal cross-reactivity), and linearity/recovery in representative matrices. Refer to the kit insert for metrics applicable to this item.
  • Low background is achieved through carefully optimized blocking and wash buffers; stabilizers may be present to preserve enzyme/antibody functionality.
• Documentation and traceability
  • For acceptance criteria (e.g., OD ranges for controls, standard concentrations, plate map), consult the kit Certificate of Analysis and Instruction Manual accompanying the specific lot.

Note: No additional item-specific acceptance values (e.g., standard range, LOD/LOQ, precision) are provided here; refer to the CoA/Spec Sheet.

Reaction and Applications

• Item-specific intent (from Product Data)
  • Human Adiponectin ELISA Kit intended for quantitative measurement of adiponectin in research samples.
• Assay principle (general ELISA overview; not item-specific)
  • Most adiponectin ELISA kits employ a sandwich format: immobilized capture antibody binds adiponectin from the sample; a detector antibody (often enzyme-conjugated, or followed by an enzyme-labeled secondary) forms an immune complex; addition of chromogenic substrate (e.g., TMB) yields a colorimetric signal proportional to analyte concentration.
  • Quantification is achieved by comparing sample OD to a standard curve prepared from known adiponectin standards.
• Sample types (general guidance)
  • Commonly validated matrices include human serum, plasma (citrate/EDTA/heparin), and cell culture supernatants. Actual compatibility and required dilutions must be taken from the kit insert for this specific item.
• Practical tips (general)
  • Equilibrate all reagents to room temperature before use, unless otherwise specified.
  • Use calibrated, multichannel-compatible pipettes for consistent dispensing; avoid edge effects by uniform incubation and plate sealing.
  • Prepare fresh wash buffer and ensure adequate wash cycles to minimize background.
  • Run standards, blanks, and quality controls in duplicate or triplicate; include spiked recovery controls when validating a new matrix.

All procedural steps (incubation times/temperatures, wash cycles, substrate/stop timing, wavelength for reading) must follow this kit’s Instruction Manual.

Reaction Conditions

Not applicable as a chemical reaction section. However, assay performance in ELISA depends on precisely controlled incubation and development conditions.

• Item-specific parameters: Not specified for this item; refer to the kit insert for incubation times, temperatures, wash cycles, substrate development time, and reading wavelength.
• General ELISA conditions (not item-specific)
  • Temperature: Room temperature incubations are common; some steps may benefit from 37°C or 2–8°C holds to optimize binding or stability, per kit design.
  • Timing: Typical binding incubations range 30–120 min; substrate development 10–30 min before stopping. Overdevelopment elevates background.
  • Wavelength: HRP/TMB stop solution is commonly read at 450 nm with a 570–650 nm reference; confirm with the manual.
  • Mixing and washing: Gentle plate shaking can improve kinetics; thorough washing (3–5 cycles, sufficient soak times) is critical for low background.

Always follow the specific conditions provided in this kit’s Instruction Manual; do not extrapolate from other ELISA formats.

Safety and Handling

Always handle ELISA kits under standard laboratory biosafety and chemical hygiene practices. This section provides general guidance; defer to the SDS and kit insert for component-specific hazards.

• Item-specific facts (from Product Data)
  • Storage: 2–8°C; shipped on wet ice.
  • GHS classification, signal word, H-statements, pictograms: Not specified for this item; refer to CoA/SDS.
• Typical kit hazards (literature/general; not item-specific)
  • Chromogenic substrates (e.g., TMB) and stop solutions (often 1–2 N H2SO4) can be irritant/corrosive.
  • Preservatives (e.g., ProClin, Kathon) in buffers may be skin/eye irritants and sensitizers.
  • Human-derived samples should be treated as potentially infectious; follow BSL-2 precautions.
• PPE
  • Lab coat, nitrile gloves, and safety glasses. Use splash protection when handling stop solutions.
• Handling and hygiene
  • Avoid skin/eye contact with substrates/stop solution; do not pipette by mouth.
  • Use calibrated pipettes; avoid generating aerosols when handling biological samples.
• Incompatibilities and special precautions
  • Keep substrates away from strong oxidizers/reductants and from light to prevent degradation.
  • Do not mix components from different lots unless specified by the manufacturer.
• First aid (general guidance)
  • Skin/eye contact: Rinse with water for ≥15 min; remove contaminated clothing; seek medical attention as needed.
  • Ingestion/inhalation: Seek medical attention; provide SDS of involved component.

Consult the SDS for each bottle/vial for definitive GHS and response instructions.

Solvent Selection

Not typically applicable. This product is a complete ELISA kit comprising aqueous buffers and biological reagents rather than a reagent requiring separate solvent selection.

• Practical note
  • Use only the diluents and wash buffers supplied or specified in the kit insert. Substituting alternative solvents (organic or aqueous) can affect binding kinetics, enzyme activity, and color development.
  • If matrix dilutions are needed (e.g., for serum/plasma/tissue culture media), follow the recommended sample diluent and maximum sample matrix percentage per the kit insert.

Storage and Reconstitution

• Item-specific facts (from Product Data)
  • Storage conditions: Store at 2–8°C.
  • Shipping: Wet ice.
  • Reconstitution instructions for lyophilized components: Not specified for this item; refer to the kit insert.
• General guidance (not item-specific)
  • Upon receipt, inventory all components, record lot numbers/expiry, and store immediately at 2–8°C. Do not freeze unless explicitly instructed for a particular component.
  • Protect light-sensitive reagents (e.g., TMB substrate) from light. Keep enzyme conjugates and standards tightly capped; avoid microbial contamination.
  • If lyophilized standards are included, reconstitute with the exact volume and diluent specified, mix gently (no vigorous vortexing for proteins), and allow complete dissolution before preparing serial dilutions.
  • Avoid repeated freeze–thaw of standards/controls; if aliquoting is recommended by the insert, follow that guidance using low-bind tubes.
  • Before use, equilibrate components to room temperature as directed to minimize condensation and improve pipetting consistency.

For component stability after opening and any short-term storage of prepared working solutions, follow the times and temperatures specified in the kit’s Instruction Manual.

Structure and Identity

This product is an immunoassay kit (Human Adiponectin ELISA Kit), not a single defined chemical compound.

• Item-specific facts (from Product Data)
  • Product type: Enzyme-linked immunosorbent assay (ELISA) kit for human adiponectin detection
  • SKU: H981627
  • Category: Protein-related assay kit
  • No small-molecule identifiers are applicable.
• Chemical identifiers
  • Molecular formula: Not applicable for a kit containing multiple reagents
  • Molecular weight: Not applicable for a kit-level entry
  • SMILES, InChIKey, CAS, CID: Not applicable/not provided

2D/functional description

• The kit comprises multiple biological reagents (e.g., capture/detection antibody-coated components, standards, buffers, substrate), collectively enabling immunoenzymatic quantification of human adiponectin. There is no single molecular structure to depict.

Notes

• For component-level identities and lot-specific details (e.g., antibody clones, sequences, concentrations), refer to the kit insert and Certificate of Analysis (CoA).

Synthetic Utility

Not applicable. This product is an ELISA kit designed for biomolecule quantification rather than a synthetic reagent or building block.

• For users seeking adiponectin-related reagents for biochemical modification or assay development, consider purified recombinant proteins, capture/detection antibodies, or labeled secondary antibodies as appropriate.

Refer instead to the Reaction & Applications and Application Protocols sections for assay-related guidance.

Target Specificity

• Item-specific facts (from Product Data)
  • Target antigen: Human adiponectin (AdipoQ).
  • Species reactivity: Human (inferred from product name). No cross-reactivity data provided.
  • Antibody clone, isotype, epitope, and cross-reactivity: Not specified for this item; refer to the kit insert/CoA.
• General considerations (not item-specific)
  • Adiponectin exists in multiple oligomeric forms; some assays target total adiponectin while others bias toward specific multimers. Verify the kit’s specificity profile (e.g., epitope, oligomer sensitivity) in the Instruction Manual.
  • Potential matrix interferences (lipemia, hemolysis, high biotin) can influence assay response depending on assay architecture.

For definitive specificity and interference testing results, consult the kit documentation for this SKU.

Häufig gestellte Fragen

What does for enzyme immunoassay(ELISA) mean?

for enzyme immunoassay(ELISA) indicates the product has been validated for use in immunoassays such as ELISA, IF, IHC and flow cytometry. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Bioactive mean?

Bioactive indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at 2–8 °C. Refrigerated storage is required to maintain the specified shelf life.

How is this product shipped?

This product ships chilled on wet ice. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.