Hyaluronidase（specificity for hyaluronate sodium）, CAS No.37326-33-3

CAS: 37326-33-3 Cat. No.: H766309 EC Number: 253-464-3

GRADE & PURITY Recombinant, EnzymoPure™, expressed in E.coli;≥1000 U/mg enzyme powder; ≥2000 U/mg protein

Expression system

E.coli & Pichia pastoris

Bioactivity

≥1000 U/mg enzyme powder; ≥2000 U/mg protein

Overview

Microbial derived hyaluronic acid (HA) degrading enzymes belong to polysaccharide lyases and are commonly referred to as HA lyases (EC 4.2.2.1). These enzymes break the β -1,4-glycosidic bond between N-acetylglucosamine (GlcNAc) and glucuronic acid (GlcUA) in the HA sugar chain through a β - elimination reaction, and produce an unsaturated double bond at the C4 and C5 positions of the glucuronic acid. This double bond has characteristic absorption at 232 nm and can be conveniently used for oligosaccharide product analysis and detection. HAase has been found in various bacteria, such as Proteus, Bacteroides fragilis, Streptococcus, Staphylococcus, Peptostreptococcus, Propionibacterium, Streptomyces, Clostridium, and Vibrio Wait. Many HAases have both chondroitin sulfate/chondroitin sulfate degradation activity, and these HAases are often classified as chondroitin sulfate CSase AC. Many HAases initially exhibit endonuclease characteristics when degrading substrates, randomly binding and cleaving HA/CS polysaccharide chains, followed by exonuclease characteristics. Therefore, rapid accumulation of unsaturated disaccharide products is often observed during substrate degradation, while the proportion of other high degree of polymerization oligosaccharides remains low. In our product, to avoid confusion, we classify HAases with both HA and CS degradation activities as CSase AC. We can provide various HAases with different specific enzyme activities and substrate structure selectivity according to customer needs. Here, HAase refers to HA specific degrading enzymes from Streptomyces hyalurolyticus and others, which can be used as specific tool enzymes for HA analysis and purity detection in biological samples.

Specifications

Product Name

Hyaluronidase（specificity for hyaluronate sodium）, CAS No.37326-33-3

Grade

Recombinant, EnzymoPure™

Specifications & Purity

Recombinant, EnzymoPure™, expressed in E.coli;≥1000 U/mg enzyme powder; ≥2000 U/mg protein

Bioactivity

≥1000 U/mg enzyme powder; ≥2000 U/mg protein

CAS

37326-33-3

Enzyme Commission Number

EC 4.2.2.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

expressed in E.coli;≥1000 U/mg enzyme powder; ≥2000 U/mg protein

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Unit definition

One unit degrades 0.75 μg of polysaccharide hyaluronic acid per minute at 37 °C and pH 6.0, determined by turbidimetric absorbance measurement at 600 nm.

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Indoles and derivatives
      • Subclass Indolyl carboxylic acids and derivatives

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Indoles and derivatives
• Subclass: Indolyl carboxylic acids and derivatives
• Intermediate Tree Nodes: Not available
• Direct Parent: Indolyl carboxylic acids and derivatives
• Alternative Parents: Indoles Tertiary alkylarylamines Medium-chain fatty acids Heterocyclic fatty acids Amino fatty acids Benzenoids Amino acids Monocarboxylic acids and derivatives Enamines Carboxylic acids Azacyclic compounds Organic oxides Hydrocarbon derivatives Hydrobromides Carbonyl compounds
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Indolyl carboxylic acid derivative - Indole - Medium-chain fatty acid - Tertiary aliphatic/aromatic amine - Amino fatty acid - Heterocyclic fatty acid - Fatty acyl - Benzenoid - Amino acid or derivatives - Amino acid - Tertiary amine - Carboxylic acid derivative - Carboxylic acid - Azacycle - Enamine - Monocarboxylic acid or derivatives - Amine - Organonitrogen compound - Organooxygen compound - Hydrobromide - Hydrocarbon derivative - Organic oxide - Carbonyl group - Organic oxygen compound - Organic nitrogen compound - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as indolyl carboxylic acids and derivatives. These are compounds containing a carboxylic acid chain (of at least 2 carbon atoms) linked to an indole ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

References

1. Yidan Chen, Tiantian Feng, Xiaohong Zhu, Yuting Tang, Yao Xiao, Xiuhua Zhang, Sheng-Fu Wang, Dong Wang, Wei Wen, Jichao Liang, Huayu Xiong.  (2024)  Ambient Synthesis of Porphyrin-Based Fe-Covalent Organic Frameworks for Efficient Infected Skin Wound Healing.  BIOMACROMOLECULES,      [PMID:38720431] [10.1021/acs.biomac.4c00261]

2. Mengyao Qi, Yi Ke, Yifei Li, Peng Li, Huakuo Zhou, Xia Zhang, Jieshi Chen, Jun Meng.  (2024)  Au@Pt nanozyme-based smart hydrogel for visual detection of hyaluronic acid.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2024.137144]

3. Tao Meng, Zhengyu Zhao, Mengqiang Song, Rui Wang, Xu Xu, Min Yang, Weihua Li, Shiyong Song.  (2025)  Carboxybetaine modified chitosan as viscosupplementation for osteoarthritis therapy.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:40090277] [10.1016/j.ijbiomac.2025.142155]

4. Yu Pang, Jie Lv, Chengcai He, Chengda Ju, Yulong Lin, Cong Zhang, Meng Li.  (2024)  Covalent organic frameworks-derived carbon nanospheres based nanoplatform for tumor specific synergistic therapy via oxidative stress amplification and calcium overload.  JOURNAL OF COLLOID AND INTERFACE SCIENCE,      [PMID:38330663] [10.1016/j.jcis.2024.01.217]

5. Siying Huang, Simeng Xiao, Xuehao Li, Ranran Tao, Zhangwei Yang, Ziwei Gao, Junjie Hu, Yan Meng, Guohua Zheng, Xinyan Chen.  (2024)  Development of Dual-Targeted Mixed Micelles Loaded with Celastrol and Evaluation on Triple-Negative Breast Cancer Therapy.  Pharmaceutics,  16  (9): (1174).  [PMID:39339211] [10.3390/pharmaceutics16091174]

6. Shiyi Yao, Hui Yuan, Luqi Yang, Yin Zhang, Hanyu Wang, Renxuan Li, Tingjun Ye, Wenguo Cui, Lei Wang.  (2025)  Downregulation of the PI3K/AKT/mTOR/MMP-13 pathway for promoting interface healing via lubricating microspheres.  Acta Biomaterialia,      [PMID:39761787] [10.1016/j.actbio.2025.01.001]

7. Jing Zhang, Yunfeng Zeng, Yongyuan Heng, Yu Shen, Ximeng Sun, Yijia Wang, Haiping Zheng, Ming Zeng, Ziyi Yu.  (2024)  Enzyme-Assisted Activation Technique for Producing Versatile Hydrogel Microparticle Scaffolds with High Surface Chemical Reactivity.  ADVANCED FUNCTIONAL MATERIALS,      [PMID:] [10.1002/adfm.202400858]

8. Jingnan Li, Chengyan Luo, Tingyu Sun, Yinglin Zhou, Xinchang Huang, Dezhou Wu, Xirui Luo, Chao Zeng, Huanan Li.  (2024)  Hypoxia-Specific Metal–Organic Frameworks Augment Cancer Immunotherapy of High-Intensity Focused Ultrasound.  ACS Nano,      [PMID:38949962] [10.1021/acsnano.4c02921]

9. Huiping Yang, Yinning Lin, Qian Mo, Zhaoquan Li, Fan Yang, Xinchun Li.  (2024)  Monitoring Enzymatic Reaction Kinetics and Activity Assays in Confined Nanospace.  ANALYTICAL CHEMISTRY,      [PMID:39024010] [10.1021/acs.analchem.4c01901]

10. Shuo Yu, Lu Zhang, Yanshen Yang, Meijuan Wang, Tingting Liu, Wenwen Ji, Yang Liu, Hao Lv, Yang Zhao, Xi Chen, Tinghua Hu.  (2024)  Polydopamine-Based Resveratrol-Hyaluronidase Nanomedicine Inhibited Pancreatic Cancer Cell Invasive Phenotype in Hyaluronic Acid Enrichment Tumor Sphere Model.  ACS Pharmacology & Translational Science,      [PMID:38633596] [10.1021/acsptsci.3c00304]

11. Xin Jin, Chengxiong Wei, Kai Li, Peinan Yin, Chengwei Wu, Wei Zhang.  (2024)  Polyphenol-mediated hyaluronic acid/tannic acid hydrogel with short gelation time and high adhesion strength for accelerating wound healing.  CARBOHYDRATE POLYMERS,      [PMID:39048222] [10.1016/j.carbpol.2024.122372]

12. Dandan Wei, Guanhua Jiao, Yinghua Tao, Liuxin Yang, Tao Liu, Fengya Jing, Tianzhu Zhang.  (2025)  Polypropylene Mesh Coated with Dual Cross-Linked Hyaluronic Acid/Polyvinyl Alcohol Composite Hydrogel with Antiadhesion and Angiogenesis Properties for Abdominal Wall Repair.  Advanced Materials Technologies,      [PMID:] [10.1002/admt.202401786]

13. Dandan Wei, Yulin Huang, Min Liang, Liuxin Yang, Guanhua Jiao, Yinghua Tao, Li Xu, Tianzhu Zhang, Zhenling Ji.  (2024)  Polypropylene mesh coated with hyaluronic acid/polyvinyl alcohol composite hydrogel for preventing bowel adhesion.  INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,      [PMID:38705326] [10.1016/j.ijbiomac.2024.132061]

14. Chao-fan He, Tian-hong Qiao, Xu-chao Ren, Mingjun Xie, Qing Gao, Chao-qi Xie, Peng Wang, Yuan Sun, Huayong Yang, Yong He.  (2025)  Printability in Multi-material Projection-Based 3-Dimensional Bioprinting.  Research,      [PMID:40041038] [10.34133/research.0613]

15. Zhu Dan, Zhong Hanyan, Shi Jingzhan, Liu Qiang, Wang Yiping.  (2025)  Ratiometric surface-enhanced Raman scattering quantification of extracellular matrix metalloproteinase-2 activity for tumor diagnosis.  ANALYTICAL AND BIOANALYTICAL CHEMISTRY,      [PMID:39966175] [10.1007/s00216-025-05792-5]

16. Yingying Chen, Dan Zhu, Hanyan Zhong, Zhixing Gan, Shenfei Zong, Zhuyuan Wang, Yiping Cui, Yiping Wang.  (2024)  Ultrasensitive Detection of Matrix Metalloproteinase 2 Activity Using a Ratiometric Surface-Enhanced Raman Scattering Nanosensor with a Core–Satellite Structure.  ACS Applied Materials & Interfaces,      [PMID:38204415] [10.1021/acsami.3c15344]

17. Shangting Du, Lingyu Li, Junhao Kou, Tianyi Zhu, Zhenyi Song, Yonghua Zhan, Daocheng Wu, Wenhua Zhan.  (2025)  Blackberry-Like Doxorubicin Loaded Hyaluronic Acid/Zinc Phthalocyanine Loaded Mesoporous Silica Nanocomposites for Long-Term Tumor Photodynamic and Chemotherapy Synergistic Therapy.  International Journal of Nanomedicine,      [PMID:40692537] [10.2147/IJN.S530041]

18. Jiancong Ni, Xiaochun Xu, Bifang Yang, Liyang Liu, Weiqiang Yang, Xiaoping Chen, Guiping Chen, Qingxiang Wang, Zhenyu Lin.  (2025)  Indicator-Free Detection of Hyaluronidase Based on a Conductivity-Regulated Bipolar Electrochemiluminescence Platform.  ANALYTICAL CHEMISTRY,      [PMID:40827572] [10.1021/acs.analchem.5c03409]

19. Haojie Qiu, Jingyan Zhu, Kaikai Zhang, Xinnan Wang, Yaoting Chu, Feng Zhang, Xuefeng Yang.  (2025)  Dual Drug-Loaded Hyaluronate-Based Self-Adaptable Hydrogel for Diabetic Infected Wound Healing.  BIOMACROMOLECULES,      [PMID:40845349] [10.1021/acs.biomac.5c00712]

20. Wenjun Xue, Xiaoyu Kuang, Xuanzhen Meng, Boyu Sun, Zijun Zhao, Guizhao Liang.  (2025)  Identification and inhibition mechanism of novel collagen-derived hyaluronidase inhibitory peptides.  Food Bioscience,      [PMID:] [10.1016/j.fbio.2025.106263]