Hydroxyurea (NSC-32065) - Moligand™, 10mM in DMSO , Ribonucleoside-diphosphate reductase RR1 inhibitor, CAS No.127-07-1, Ribonucleoside-diphosphate reductase RR1 inhibitor

CAS: 127-07-1 Cat. No.: H408867 Molecular Weight: 76.05 Beilstein Registry Number: 1741548 EC Number: 204-821-7

GRADE & PURITY Moligand™, 10mM in DMSO

Synonyms

NCI-C04831, Hydroxycarbamide | 1-hydroxyurea

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Overview

Information

Hydroxyurea (NSC-32065) Hydroxyurea (NSC-32065, NCI-C04831, Hydroxycarbamide) is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase . Hydroxyurea activates apoptosis and autophagy . Hydroxy
In vitro

hydroxyurea can inhibit HIV-1 replication. In vitro experiments have shown that the 90% inhibitory concentration (IC90) of hydroxyurea for laboratory strains of HIV-1 in activated PBMC is 0.4 mM. Hydroxyurea was also found to be synergistic with the nucleoside reverse transcriptase inhibitor didanosine and to inhibit HIV-1 replication in activated PBMC; this inhibition may be due to a reduction in deoxynucleoside triphosphate pool sizes. Hydroxyurea has been shown to sensitize didanosine-resistant mutants.hydroxyurea has demonstrated activity in the treatment of sickle cell anemia by increasing the production of fetal hemoglobin, which reduces hemolysis in patients with this disease. Hydroxyurea exerts its cytostatic effect through inhibition of ribonucleotide reductase—the rate-limiting enzyme responsible for the conversion of ribonucleotides to deoxyribonucleotides, which are essential for DNA synthesis. As a result, cellular division is arrested in the S phase.

In vivo

Cell Data

cell lines：

Concentrations：30 μM

Incubation Time：96 h

Powder Purity:≥97%

Specifications

Synonyms

NCI-C04831, Hydroxycarbamide | 1-hydroxyurea

Specifications & Purity

Moligand™, 10mM in DMSO

Biochemical and Physiological Mechanisms

Hydroxyurea (NSC-32065, NCI-C04831, Hydroxycarbamide) is an antineoplastic agent that inhibits DNA synthesis through the inhibition of ribonucleoside diphosphate reductase. Hydroxyurea activates apoptosis and autophagy. Hydroxyurea is used to treat HIV in

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Action Type

INHIBITOR

Mechanism of action

Ribonucleoside-diphosphate reductase RR1 inhibitor

Product Properties

• ALogP: -1.8

Names and Identifiers

• Isomeric SMILES: C(=O)(N)NO
• WGK Germany: 3
• RTECS: YT4900000
• UN Number: 2811
• Packing Group: I
• Molecular Weight: 76.05
• Beilstein: 1741548
• Reaxy-Rn: 1741548
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=1741548&ln=

Associated Targets(Human)

RRM1 Tclin Ribonucleoside-diphosphate reductase large subunit (1 Activities)

Discover Target: RRM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

RRM2 Tclin Ribonucleoside-diphosphate reductase subunit M2 (1 Activities)

Discover Target: RRM2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Chemical and Physical Properties

• Melt Point(°C): 145°C

References

1. Zhongyi Cong, Jingyuan Jiang, Mengyuan Li, Chenqi Yan, Jiayin Li, Xinmin Zhang.  (2025)  Fmp45 promotes Rad53 dephosphorylation via Ptc2 interaction to attenuate checkpoint signaling and maintain genome stability.  Journal of Genetic Engineering and Biotechnology,      [PMID:41386832] [10.1016/j.jgeb.2025.100567]

2. Kai Wang, Wentao Zhou, Xiangyu Jin, Xuwei Shang, Xiaomei Wu, Lijuan Wen, Sufen Li, Yiling Hong, Jia Ke, Yichong Xu, Hong Yuan, Fuqiang Hu.  (2023)  Enhanced brain delivery of hypoxia-sensitive liposomes by hydroxyurea for rescue therapy of hyperacute ischemic stroke.  Nanoscale,  15  (27): (11625-11646).  [PMID:37377137] [10.1039/D3NR01071F]

3. Yafeng Jin, Fangming Han, Xiaobo Li, Haibo Shang, Huan Zhang, Li Yang, Tao Wang, Jun Chen, Guangri Xu, Cheng-Xing Cui.  (2024)  A novel low oxidation potential hydroxyurea sensor utilizing flower-shaped Zn and N co-doped porous carbon coupled with graphene oxide via one-step electrodeposition.  MICROCHEMICAL JOURNAL,      [PMID:] [10.1016/j.microc.2024.110714]

4. Juntao Hu, Yibing Huang, Hao Hao, Pujing Tian, Yinuo Yin, Yuting He, Fengjie Hao, Wantong Jiang, Yanping Zhang, Youzhong Wan, Quan Luo.  (2025)  Bioinspired programmed antibiofilm strategies for accelerated wound healing via spatiotemporally controlled enzyme nanoreactors.  JOURNAL OF CONTROLLED RELEASE,      [PMID:40032010] [10.1016/j.jconrel.2025.113582]

5. Wanying Cheng, Cenzhu Wang, Meican Ma, Yu Zhou.  (2025)  Hydroxyurea mitigates diabetic kidney disease through mTOR-S6K signaling pathway in STZ-induced diabetic mice.  Frontiers in Cell and Developmental Biology,      [PMID:39911324] [10.3389/fcell.2025.1529901]

6. Weichen Dong, Wenxin Zhang, Li Huang, Lulu Xiao, Yuanfei Luo, Linying Yuan, Yike Jiang, Anyu Liao, Hongting Zhao, Zhihui Liu, Jia Wang, Ying Zhao, Yulong Cai, Mengna Peng, Dong Yang, Yi Xie, Kuanyu Li, Wusheng Zhu.  (2026)  Restoring cardiolipin homeostasis mitigates cerebral ischemia–reperfusion injury by suppressing ATG5-mediated neuronal autophagy-dependent ferroptosis.  Journal of Advanced Research,      [PMID:] [10.1016/j.jare.2026.03.014]