IGJ Mouse mAb - Primärer Antikörper, specific to IGJ

Cat. No.: Ab109641

GRADE & PURITY Validated, ExactAb™, See COA

Application

IHC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

OTI3H7

Conjugation

Unconjugated

Übersicht

Mouse anti Human IGJ Antibody, Monoclonal (OTI3H7), could be used for WB, IHC and so on.

Specifications

Product Name

IGJ Mouse mAb - Primärer Antikörper, specific to IGJ

Synonyme

IGCJantibody | IGJ_HUMAN antibody | Immunoglobulin J chain antibody | Immunoglobulin J polypeptide linker protein for immunoglobulin alpha and mu polypeptides antibody | immunoglobulin joining chain antibody | JCH antibody | JCHAIN antibody | Joining chai

Spezifikationen & Reinheit

ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

Serves to link two monomer units of either IgM or IgA. In the case of IgM, the J chain-joined dimer is a nucleating unit for the IgM pentamer, and in the case of IgA it induces dimers and/or larger polymers. It also helps to bind these immunoglobulins to

Wirtsspezies

Maus

Spezifität

IGJ

Immunogen

Recombinant full length protein of human IGJ.

Positive Kontrolle

WB: HEK293T transfected with pCMV6-ENTRY IGJ cDNA for 48 hrs; Human testis, omentum, uterus, breast, brain, ovary, thyroid gland, colon lysates. IHC: Human bladder carcinoma, lymphoma tissues.

Konjugation

Unconjugated

Note

ExactAb™, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: IGJ Mouse mAb (Ab109641)

IGJ Mouse mAb (Ab109641) - Western Blot
All lanes: IGJ Mouse mAb (Ab109641) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 23 kDa
Exposure time: 100.0 s

Western Blot: IGJ Mouse mAb (Ab109641)

IGJ Mouse mAb (Ab109641) - Western Blot
All lanes: IGJ Mouse mAb (Ab109641) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/40000 dilution
Predicted band size: 18 kDa
Observed band size: 23 kDa
Exposure time: 18.0 s

IHC: IGJ Mouse mAb (Ab109641)

IGJ Mouse mAb (Ab109641) - IHC
Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using IGJ Mouse mAb (Ab109641). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab109641).

IHC: IGJ Mouse mAb (Ab109641)

IGJ Mouse mAb (Ab109641) - IHC
Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using IGJ Mouse mAb (Ab109641). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Ab109641).

Anwendung

• Anwendung: Dilution info
• WB:

  1/2000;

• IHC:

  1/50

Zugehörige Ziele (menschlich)

JCHAIN Tbio Immunoglobulin J chain (0 Activities)

Discover Target: JCHAIN

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (IGJ Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.