Iscalimab (anti-CD40) - Primärer Antikörper, specific to CD40, Human IgG1, Antibody of CD40, Antibody of CD40

CAS: 2031153-61-2 Cat. No.: Ab175491

GRADE & PURITY Animal Free, Carrier Free, Recombinant, Validated, ExactAb™, Low Endotoxin, Azide Free, ≥95%(SDS-PAGE&SEC-HPLC)

Application

Animal Model, ELISA, Flow Cytometry, Functional Assay

Host species

Mensch

Reactive species

Cynomolgus-Affe, Mensch, Rhesusaffe

Isotype

Human IgG1

Conjugation

Unconjugated

Übersicht

Iscalimab (anti-CD40) is a non-depleting IGg1 monoclonal antibody targeting CD40 (KD: 0.3 nM). Iscalimab (anti-CD40) can be used for research of Graves' hyperthyroidism and autoimmune diseases.
Purity
≥95% (SDS-PAGE&SEC)
Endotoxin Level
< 1.0EU/mg

Specifications

Product Name

Iscalimab (anti-CD40) - Primärer Antikörper, specific to CD40, Human IgG1, Antibody of CD40

Synonyme

AI326936 antibody | B cell associated molecule CD40 antibody | B cell surface antigen CD40 antibody | B cell-associated molecule antibody | B-cell surface antigen CD40 antibody | Bp50 antibody | CD 40 antibody | CD40 antibody | CD40 antigen (TNF receptor

Spezifikationen & Reinheit

Carrier Free, Recombinant, ExactAb™, Low Endotoxin, Azide Free, Validated, Animal Free, ≥95%(SDS-PAGE&SEC-HPLC)

Wirtsspezies

Mensch

Spezifität

CD40

Konjugation

Unconjugated

Note

Animal Free, Azide Free, Carrier Free, ExactAb™, Low Endotoxin, Recombinant, Validated

Aktionsart

INHIBITOR

Mechanismus der Wirkung

Antibody of CD40

Isotyp

Human IgG1

Lichterketten-Typ

kappa

SDS-PAGE

27.2 kDa (Light Chain) & 52.2 kDa (Heavy Chain), under reducing conditions; 201.2 kDa, under non-reducing conditions.

Verfahren zur Reinigung

Protein A purified

CAS

2031153-61-2

Lagerung und Versand

Form

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Store at -80℃ for 24 months. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 with Iscalimab (anti-CD40) (Ab175491) conjugated with biotin (right panel) compared with Human IgG (Biotin) (Ab180314) - Isotype Control (left panel) and detected with SA-PE (rp156150). 

ELISA: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - ELISA
Immobilized Recombinant Human CD40 protein (rp183577) at 1.0 μg/mL can bind Iscalimab (anti-CD40) (Ab175491) with the EC₅₀ of 35.88 ng/mL.

SEC: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - SEC
The purity of Iscalimab (anti-CD40) (Ab175491) is more than 95% verified by HPLC.

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of Raji cells labelling CD40 (red) with Iscalimab (anti-CD40) (Ab175491). Goat Anti-Human IgG (PE) (Ab175838) at a dilution of 1/1000 was used as the secondary antibody. Blue - Isotype control, human IgG (Ab170213). Black - Unlabelled control, cells without incubation with primary antibody.

Flow Cytometry: Iscalimab (anti-CD40) (Ab175491)

Iscalimab (anti-CD40) (Ab175491) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood lymphocytes labelling CD40 (red) with Iscalimab (anti-CD40) (Ab175491) conjugated with biotin and detected with SA-PE (rp156150). Blue - Isotype control, Human IgG (Biotin) (Ab180314). Black - Unlabelled control, cells without incubation with primary antibody.

Zugehörige Ziele (menschlich)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (0 Activities)

Discover Target: CD40

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

CD40 Tchem Tumor necrosis factor receptor superfamily member 5 (9 Activities)

Discover Target: CD40

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 2031153-61-2.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Iscalimab (anti-CD40)); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.