Isoamylase from Pseudomonas sp., CAS No.9067-73-6

CAS: 9067-73-6 Cat. No.: I1438585 EC Number: 232-956-1

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥120 U/mg protein；~200 U/mL

Accession #

P26501

Bioactivity

≥120 U/mg protein

Overview

Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and analytical testing applications. Isoamylase, Fructanase and Amyloglucosidase are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016. 

PROPERTIES 

1. ELECTROPHORETIC PURITY: 

- Single band on SDS-gel electrophoresis (MW = 71,500). 

- Single major band on isoelectric focusing (pI = 5.0). 

2. SPECIFICITY: 

Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins. 

3. PHYSICOCHEMICAL PROPERTIES: 

Recommended conditions of use are at pH 4.0 and up to 40°C. 

pH Optima: 4.0-5.0 

pH Stability: 3.5-6.0 (16 h, 4°C) 

Temperature Optima: 50°C 

Temperature Stability: <45°C (pH 4.0, 15 min) 

4. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES: 

Recommended for use in AOAC 2000.11 (polydextrose in food) 

5. PRODUCT DETAILS: 

The enzyme is supplied as an ammonium sulphate suspension containing 0.02% sodium azide and should be stored at 4°C. This enzyme is very unstable to freezing and thawing. DO NOT FREEZE. It is recommended that all buffers used for dilution contain BSA (1 mg/mL). Swirl to mix the enzyme immediately prior to use.

6. EXPERIMENTAL DATA:  

Specifications

Product Name

Isoamylase from Pseudomonas sp., CAS No.9067-73-6

Synonyms

debranching enzyme | glycogen 6-alpha-D-glucanohydrolase | iam

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥120 U/mg protein；~200 U/mL

Biochemical and Physiological Mechanisms

Hydrolysis of (1->6)-alpha-D-glucosidic branch linkages in glycogen, amylopectin and their beta-limit dextrins.

Bioactivity

≥120 U/mg protein

Accession #

P26501

Predicted molecular weight

71.5 kDa

CAS

9067-73-6

Enzyme Commission Number

EC 3.2.1.68

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥120 U/mg protein；~200 U/mL

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term. Upon receipt, it is recommended to aliquot. DO NOT FREEZE.

Unit definition

One Unit of isoamylase activity is defined as the amount of enzyme required to release one μmole of reducing sugar per minute from oyster glycogen (10 mg/mL) in sodium acetate buffer (100 mM), pH 4.0 at 40°C. One Unit as defined here is approximately equa

References

1. Min Li, Shanshan He, Sifan Yang, Yunguang Wang, Huishan Shen, Xingli Liu, Hongwei Wang, Yanyan Zhang, Hua Zhang.  (2025)  Effect of electron beam irradiation on the hierarchical structure and physicochemical properties of starch isolated from oat grains.  FOOD RESEARCH INTERNATIONAL,      [PMID:41174371] [10.1016/j.foodres.2025.117265]