K63-Linked Tetra-Ubiquitin - BioReagent,for protein analysis,≥95%(WB),See COA

Cat. No.: K1518273

GRADE & PURITY BioReagent, for Protein analysis, ≥95%(WB), See COA

Synonyms

K63 Tetra-Ubiquitin | K63-Linked Tetra-Ubiquitin | K63-Linked Tetra-Ubiquitin (Ub4)

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Application

846

Overview

  Polyubiquitylation is a post-translational modification that regulates protein stability and protein-protein interactions. The type of polyubiquitin linkage determines the fate of the modified protein (s). Polyubiquitylation through K63 linkages has recently become the focus of intense study. Notably, the topology of this linkage and its role in cellular processes differ significantly from those of K48-linked chains. K63-linked polyubiquitin appears to function in endocytic trafficking, DNA repair, neurodegeneration, and other pathways. 

  These tetra-ubiquitin chains are generated by enzymatically linking wild-type ubiquitin through lysine 63. The most distal ubiquitin contains a lysine-to-arginine substitution at position 63, limiting further chain extension.

  This construct is a useful substrate for identifying and characterizing deubiquitinating enzymes (DUBs) that cleave K63 linkages, and for structural and binding studies involving ubiquitin-associated domains (UBA) or ubiquitin-interacting motifs (UIMs).

Application

Investigation of DUB linkage specificity

Product Information

1. Species: Human

2. Source: E. coli

3. Tag: None

4. Purity:≥ 95% by Western blot analysis

5. Molecular Weight: 34,233.4 Da

6. Physical State: Liquid

7. Buffer: 20 mM Tris-HCl, pH 7.5, 0.15 M NaCl, 1 mM EDTA

8. Quantity: 25 μg

9. Solubility: >1 mg/mL

10. Storage: -80℃. Avoid repeated freeze/thaw cycles.

Western blot analysis 

RP-HPLC Profile

Specifications

Synonyms

K63 Tetra-Ubiquitin | K63-Linked Tetra-Ubiquitin | K63-Linked Tetra-Ubiquitin (Ub4)

Specifications & Purity

BioReagent, for protein analysis, ≥95%(WB), See COA

Stability And Storage

Store at -80℃ long term (12 months)

Storage

Store at -80°C, Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

BioReagent, for Protein analysis

Purity

≥95%(WB)