Kindlin 2 Mouse mAb - Primärer Antikörper, specific to FERMT2

Cat. No.: Ab112146

GRADE & PURITY Validated, KO Validation, ExactAb™, See COA

Application

IF/ICC, IHC, IP, WB

Host species

Maus

Reactive species

Affe, Allerdings, Maus, Mensch, Rat

Isotype

Mouse IgG2a

Clone Number

OTI14A11

Conjugation

Unconjugated

Übersicht

Mouse anti Human Kindlin 2 Antibody, Monoclonal (OTI14A11), could be used for WB, IHC, IF/ICC, IP and so on.

Specifications

Product Name

Kindlin 2 Mouse mAb - Primärer Antikörper, specific to FERMT2

Synonyme

FLJ34213 | FLJ44462 | UNC112 | FERM2_HUMAN | Fermitin family homolog 2 | Fermt2 | KIND2 | Kindlin-2 | MIG-2 | MIG2 | Mitogen-inducible gene 2 protein | PH domain-containing family C member 1 | Pleckstrin homology domain containing family C (with FERM doma

Spezifikationen & Reinheit

KO Validation, ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

Participates in the connection between ECM adhesion sites and the actin cytoskeleton and also in the orchestration of actin assembly and cell shape modulation. Recruits migfilin (FBLP1) protein to cell-ECM focal adhesion sites.

Wirtsspezies

Maus

Spezifität

FERMT2

Immunogen

Full-length protein expressed in 293T cell transfected with human FERMT2 expression vector

Positive Kontrolle

WB: Hep G2, HeLa, A549, NIH/3T3 and C6 cell lysates. IHC: Human pancreas tissues. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY FERMT2, Hep G2 cells. IP: HEK293T lysates.

Konjugation

Unconjugated

Note

ExactAb™, KO Validation, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/30000 dilution

Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 100.0 s

Western Blot: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - Western Blot
All lanes: Kindlin 2 Mouse mAb (Ab112146) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 78 kDa
Observed band size: 72 kDa
Exposure time: 30.0 s

KO: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - KO
Equivalent amounts of cell lysates (10 µg per lane) of wild-type HeLa cells (WT) and FERMT2-Knockout HeLa cells (KO) were separated by SDSPAGE and immunoblotted with Kindlin 2 Mouse mAb (Ab112146) (1/500). Then the blotted membrane was stripped and reprobed with anti-PCNA antibody as a loading control.

IF/ICC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Kindlin 2 Mouse mAb (Ab112146) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FERMT2.

IF/ICC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IF/ICC
Immunofluorescent staining of Hep G2 cells using Kindlin 2 Mouse mAb (Ab112146).

IHC: Kindlin 2 Mouse mAb (Ab112146)

Kindlin 2 Mouse mAb (Ab112146) - IHC
Immunohistochemical staining of paraffinembedded Human pancreas tissue within the normal limits using Kindlin 2 Mouse mAb (Ab112146). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min).

Anwendung

• Anwendung: Dilution info
• WB:

  1/1000-1/2000 ;

• IF/ICC:

  1/50-1/100

• IHC:

  1/50

• IP:

  2ug/500ul ;

Zugehörige Ziele (menschlich)

FERMT2 Tbio Fermitin family homolog 2 (0 Activities)

Discover Target: FERMT2

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does KO Validation mean?

KO Validation indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Kindlin 2 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.