LBA4404 Agrobacterium tumefaciens Glycerol Stock (for Transgenic Plant Construction) - BioReagent,bacteriological grade

Cat. No.: L747592

GRADE & PURITY BioReagent, bacteriological grade

Synonyms

Agrobacterium LBA4404 | LBA4404 agrobacterium tumefaciens glycerol stock for construction of transgenic plant | LBA4404 Agrobacterium tumefaciens

Storage

Store at -80°C,Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

Application

586

Overview

The LBA4404 strain has an Ach5-type background, with a screening marker—the rifampicin resistance gene Rif—in its nuclear genome. To facilitate transformation operations, this strain carries an octopine-type Ti plasmid pAL4404 that lacks self-transport functionality. This plasmid contains vir genes (which are essential components for the insertion of T-DNA into the plant genome; although the T-DNA transfer function of the pAL4404 plasmid itself is disrupted, it can assist in the smooth transfer of T-DNA from the introduced binary vector). The pAL4404-type Ti plasmid contains a screening marker: Strep, which endows the LBA4404 strain with streptomycin resistance and is suitable for transgenic operations in plants such as tobacco, tomato, etc.

Product Usage: Agrobacterium tumefaciens strain

Prokaryotic Resistance: Rif+、Str+

Genotype: Ach5 (rif^R) Ti pAL4404 (strep^R) Octopine

Culture Medium: LB Medium

Culture Conditions: 28–30°C, aerobic

Product Specification: 200 μL Glycerol Stock

Strain Amplification: Take 100–200 μL of the glycerol stock, inoculate it into 3–5 mL of fresh LB medium (with Rif+ and Str+ double resistance), and incubate with shaking overnight at 28–30°C. Alternatively, you can first streak the glycerol stock on an agar plate, then pick a single colony, and incubate with shaking overnight.

Specifications

Synonyms

Agrobacterium LBA4404 | LBA4404 agrobacterium tumefaciens glycerol stock for construction of transgenic plant | LBA4404 Agrobacterium tumefaciens

Specifications & Purity

BioReagent, bacteriological grade

Stability And Storage

Store at -80℃ long term (12 months). Avoid freeze/thaw cycle.

Storage

Store at -80°C, Avoid repeated freezing and thawing

Shipped In

Dry ice packs + Cold packs

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

bacteriological grade, BioReagent