Luciferase from Photinus pyralis (firefly) , CAS No.61970-00-1

CAS: 61970-00-1 Cat. No.: L465180 EC Number: 263-359-4

GRADE & PURITY Recombinant, EnzymoPure™, lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein

Overview

Product introduction

Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.

Application

The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.

Specifications

Product Name

Luciferase from Photinus pyralis (firefly) , CAS No.61970-00-1

Synonyms

Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) | firefly luciferase | luciferase (firefly luciferin) | Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing) | firefly luciferin luciferase | Photinus pyralis luciferase

Grade

Recombinant, EnzymoPure™

Specifications & Purity

EnzymoPure™, Recombinant, lyophilized powder, ≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein

Source

大肠杆菌

Note

Packaging 1mg refers to the quality of the protein.

CAS

61970-00-1

Enzyme Commission Number

1.13.12.7

Molecule Type

Enzyme

Storage and Shipping

Concentration

lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein

Storage

Store at -80°C

Shipped In

Dry ice packs + Cold packs

Unit definition

One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.

References

1. Wang Zhengwei, Lin Jiani, Tan Guisen, Pan Tingzheng, Zhang Hongtian, Liu Jiangang.  (2025)  miR-1270 suppresses glioblastoma development by transcriptional inhibition of nuclear factor IX.  MOLECULAR BIOLOGY REPORTS,  52  (1): (1-10).  [PMID:40815328] [10.1007/s11033-025-10915-3]

2. Weiwei Liu, Qiuyue Ou, Meichang Feng, Yue Li, Linghan Huang, Xuan Peng, Xiaoqiang Zhong, Guoqiang Huang, Hengbo Wang, Jinlong Guo.  (2025)  Cold-Induced Promoter Methylation Attenuates ScMYB7-Mediated Repression of the CBF Pathway: A Proposed Mechanism for Sugarcane Cold Adaptation.  PLANT CELL AND ENVIRONMENT,      [PMID:40947544] [10.1111/pce.70180]