MAFK Mouse mAb - Primärer Antikörper, specific to MAFK, Mouse IgG1

Cat. No.: Ab113994

GRADE & PURITY Carrier Free, Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

D28S786

Conjugation

Unconjugated

Übersicht

Mouse anti Human MAFK Antibody, Monoclonal (D28S786), could be used for WB, Flow and so on.

Specifications

Product Name

MAFK Mouse mAb - Primärer Antikörper, specific to MAFK, Mouse IgG1

Synonyme

Erythroid transcription factor NF E2 p18 subunit | Erythroid transcription factor NF-E2 p18 subunit | FLJ32205 | MAFK | MAFK_HUMAN | MGC71717 | Nfe2u | P18 | Transcription factor MafK | V maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian) | Er

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 0.5 mg/mL

Biochemische und physiologische Mechanismen

Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper pr

Wirtsspezies

Maus

Spezifität

MAFK

Immunogen

KLH-conjugated synthetic peptide of human MAFK.

Positive Kontrolle

WB: Jurkat, HeLa and A549 cell lysates. Flow: HeLa cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

0.5 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: MAFK Mouse mAb (Ab113994)

MAFK Mouse mAb (Ab113994) - Western Blot
All lanes: MAFK Mouse mAb (Ab113994) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 19 kDa
Exposure time: 8.2 s

Western Blot: MAFK Mouse mAb (Ab113994)

MAFK Mouse mAb (Ab113994) - Western Blot
All lanes: MAFK Mouse mAb (Ab113994) at 1/2000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution

Predicted band size: 17 kDa
Observed band size: 19 kDa
Exposure time: 100.0 s

Flow Cytometry: MAFK Mouse mAb (Ab113994)

MAFK Mouse mAb (Ab113994) - Flow Cytometry
Flow cytometric analysis of HeLa cells using MAFK Mouse mAb (Ab113994). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by MAFK Mouse mAb (Ab113994) at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF488 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Anwendung

• Anwendung: Dilution info
• WB:

  1/500-1/2000;

• Flow Cytometry:

  1/10-1/50;

Zugehörige Ziele (menschlich)

MAFK Tbio Transcription factor MafK (0 Activities)

Discover Target: MAFK

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

MAFK Tbio Transcription factor MafK (3 Activities)

Discover Target: MAFK

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MAFK Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.