MelanA Mouse mAb - Primärer Antikörper, specific to MelanA, Mouse IgG1

Cat. No.: Ab114904

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0 mg/mL

Application

IHC, IP, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

KAA343_6B7

Conjugation

Unconjugated

Übersicht

Mouse anti Human MelanA Antibody, Monoclonal (KAA343_6B7), could be used for WB, IHC, IP and so on.

Application

WB: 1/1000

IHC: 1/200

IP: 1/100

Protein Function

Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.

Specifications

Product Name

MelanA Mouse mAb - Primärer Antikörper, specific to MelanA, Mouse IgG1

Synonyme

Antigen LB39 AA antibody | Antigen LB39-AA antibody | Antigen SK29 AA antibody | Antigen SK29-AA antibody | MAR1_HUMAN antibody | MART 1 antibody | MART-1 antibody | MART1 antibody | Melan A antibody | Melan A protein antibody | Melanoma antigen recognize

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0 mg/mL

Wirtsspezies

Maus

Spezifität

MelanA

Immunogen

Recombinant protein of human MelanA.

Positive Kontrolle

WB: SK-MEL-28 lysates. IHC: Human malignant melanoma tissue. IP: SK-MEL-28 cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -80°C,Avoid repeated freezing and thawing

Verschickt in

Dry ice packs + Cold packs

Stabilität und Lagerung

Store at 2-8 ℃ (3 months). Store at -20 ℃ (12 months). Store at -80 ℃ (36 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: MelanA Mouse Antibody (Ab114904)

MelanA Mouse Antibody (Ab114904) - Western Blot
15 µg of SK-MEL-28 lysate was run on 6-18% SDS-PAGE under reducing conditions and blotted onto nitrocellulose membrane. MelanA Mouse Antibody (Ab114904) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. MLANA band was visualized using ECL Western Blotting Substrate.
Result: MelanA Mouse Antibody (Ab114904) can detect MLANA by Western blotting.

IP: MelanA Mouse Antibody (Ab114904)

MelanA Mouse Antibody (Ab114904) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of MelanA Mouse Antibody (Ab114904) with SK-MEL-28 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. MelanA Mouse Antibody (Ab114904) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG (Fc specific) was used as the secondary antibody.
Lane 1: SK-MEL-28 lysate
Lane 2: MLANA immunoprecipitated from SK-MEL-28 lysate by MelanA Mouse Antibody (Ab114904)
Lane 3: The same as Lane 2 was IgG isotype control antibody
Result: MelanA Mouse Antibody (Ab114904) can immunoprecipitate MLANA.

IHC: MelanA Mouse Antibody (Ab114904)

MelanA Mouse Antibody (Ab114904) - IHC
IHC-P analysis of malignant melanoma tissue by MelanA Mouse Antibody (Ab114904).IHC-P was performed using sections of the formalin-fixed paraffin-embedded malignant melanoma tissue. Antigen was retrieved through addition of boiling Tris/EDTA buffer pH 9 in a pressure cooker for 3 min. Endogenous peroxidase activity was quenched by incubating the sections with 3% H2O2 for 30 min at room temperature. The sections were then incubated with MelanA Mouse Antibody (Ab114904) at 5 µg/mL at room temperature for 1 h. Poly-peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Diaminobenzidine was used as the chromogen. The section was counterstained with hematoxylin. A tissue section incubated with phosphate-buffered saline followed by incubation with the secondary antibody was used as the background control. Result: Tumor cells are positively stained at the cytoplasm.

Anwendung

• Anwendung: Dilution info
• WB:

  1/1000 ;

• IP:

  1/100;

• IHC:

  1/200 ;

Zugehörige Ziele (menschlich)

MLANA Tbio Melanoma antigen recognized by T-cells 1 (0 Activities)

Discover Target: MLANA

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

How is this product shipped?

This product ships frozen with dry ice and cold packs. Unpack on arrival and transfer it to the storage condition stated above; handle dry ice with insulated gloves in a ventilated area.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?80 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (MelanA Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.