Modified Hoagland Hydroponic Medium (Nitrogen-Free, without Calcium Nitrate) - BioReagent, Suitable for plant cell culture

Cat. No.: M1524328
AVAILABLE TO ORDER
GRADE & PURITY BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. Suitable for plant cell culture ? Plant cell-culture grade — formulated for plant tissue and cell cultures. Use in plant biotechnology, callus, and suspension culture work.
Storage
Protected from light,Room temperature
Shipped In
Normal
Application
917
Size
Status
Price
Qty
250g
M1524328-250g
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$49.90
Enter a quantity for the sizes you want to add.
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Why this grade

BioReagent, Suitable for plant cell culture BioReagent,Suitable for plant cell culture for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Protected from light,Room temperature Ships Normal Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

This product is a precisely modified formulation of the classic Hoagland nutrient solution. Nitrogen and calcium nitrate are removed, while the proportions of all other nutrients remain balanced. It offers a single experimental variable, extremely low interference and excellent reproducibility. Featuring high solubility, good system stability and low precipitation tendency, it is widely used in research experiments on plant nutrition, compatible with various cultivation methods including hydroponics, and is a commonly used specialized culture solution for plant physiology studies.

Product Composition: 

Component(mg/L) 
Potassium chloride152.828
Dipotassium hydrogen phosphate trihydrate456.638
Magnesium sulfate heptahydrate246.48
Sodium ferric EDTA11.17
Boric acid1.546
Manganese sulfate monohydrate0.338
Copper sulfate0.125
Zinc sulfate monohydrate0.576
Sodium molybdate0.102

Usage Instructions:

1. Accurately weigh 0.635g of this product, add both into 1L of distilled water and stir until the powders are completely dissolved to prepare a homogeneous culture medium solution.

2. Sterilize via high-pressure steam at 116°C for 30 minutes. After sterilization, place the medium in a clean environment for natural cooling before use in plant tissue culture experiments.

Precautions:

1. Store at room temperature away from light; shelf life is 3 years.

2. This plant tissue culture medium adopts a full inorganic salt formula. A small amount of precipitate may form after sterilization.

3. This product is for research purposes only. Do not use it for medicine, clinical diagnosis or treatment, food, cosmetics or other applications. Do not store it in residential areas.

4. Our company shall not be liable for any accidents arising from improper use of this product.

Specifications

Specifications & Purity
BioReagent, Suitable for plant cell culture
Stability And Storage
Store at room temperature long term (36months). Store in the dark.
Storage
Protected from light, Room temperature
Shipped In
Normal
Grade
BioReagent, Suitable for plant cell culture

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Chemical and Physical Properties
SensitivityLight-sensitive
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View BioReagent grade guide → View Suitable for plant cell culture grade guide →

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