Mouse IgG1-kappa, anti-E8L - Isotyp-Kontrollantikörper, specific to E8L

Cat. No.: Ab213557

GRADE & PURITY Animal Free, Carrier Free, ExactAb™, Azide Free, Validated, High Performance, PBS Only, ≥95%(SDS-PAGE), 1.0 mg/mL

Application

ELISA, Flow Cytometry

Host species

Maus

Reactive species

Virus

Isotype

Mouse IgG1

Clone Number

EF12/6D11

Conjugation

Unconjugated

Übersicht

Mouse IgG1, Isotype Control Antibody (EF12/6D11), could be used for Flow Cytometry analysis, ELISA and so on.

Specifications

Product Name

Mouse IgG1-kappa, anti-E8L - Isotyp-Kontrollantikörper, specific to E8L

Synonyme

Mouse IgG1, κ Isotype Control | Purified Mouse IgG1, kappa Isotype Control | Mouse IgG1 Isotype Control Antibody | Mouse IgG1 Isotype Control mAb | Immunoglobulin G1 Fc | Mouse IgG1 Isotype Control | Isotype control mAb for mouse IgG1 | IgG1 (Mouse) Isoty

Spezifikationen & Reinheit

Animal Free,Carrier Free,ExactAb™,Azide Free,Validated,High Performance,PBS Only,≥95%(SDS-PAGE),1.0 mg/mL

Biochemische und physiologische Mechanismen

Mouse IgG1 is a subtype of immunoglobulin G antibody that is produced by B cells in response to an antigen. It is the most abundant IgG subtype in the serum and plays a crucial role in the adaptive immune response. Mouse IgG1 is composed of two heavy chai

Wirtsspezies

Maus

Spezifität

E8L

Immunogen

Recombinant protein corresponding to MPXV E8L

Positive Kontrolle

Flow Cytometry: Peripheral blood mononuclear cells; Mouse splenocytes

Konjugation

Unconjugated

Note

Animal Free, Azide Free, Carrier Free, ExactAb™, High Performance, PBS Only, Validated

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A purified

Konzentration

1.0 mg/mL

Product Line

Biologische Produkte

Lagerung und Versand

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

Bilder

Flow Cytometry: Mouse IgG1-kappa, anti-E8L (Ab213557)

Mouse IgG1-kappa, anti-E8L (Ab213557) - Flow Cytometry
Flow Cytometry analysis of human peripheral blood mononuclear cells labelling CD40 (Red) with CD40 Mouse mAb (Ab094768) conjugated with biotin at 2.0 μg/mL for 1 hour at 4°C, compared to concentration matched Mouse IgG1-kappa, anti-E8L (Ab213557) (Blue) conjugated with biotin. Detected with SA-PE (rp156150).

ICC/IF: Mouse IgG1-kappa, anti-E8L (Ab213557)

Mouse IgG1-kappa, anti-E8L (Ab213557) - ICC/IF
IF analysis of HeLa cells using beta Tubulin Mouse mAb (Ab179922) (Green) compared to concentration matched Mouse IgG1-kappa, anti-E8L (Ab213557). The cells were fixed and permeabilized with 100% methanol for 5 minutes, and blocked with 2% BSA for 1 hour at room temperature. A Goat Anti-Mouse IgG H&L (FITC) (Ab179003) was used at a dilution of 1/1000. Cells were counterstained with DAPI (Blue). Images were taken on the confocal laser scanning microscope.

Anwendung

Anwendung

Dilution info

Flow Cytometry

Use at the same concentration as the detection antibody.

Häufig gestellte Fragen

What is the purity of this product, and how is it determined?

This product is supplied at ≥95% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Animal Free mean?

Animal Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (Mouse IgG1-kappa, anti-E8L); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.