NME4 Mouse mAb - Primärer Antikörper, specific to NME4

Cat. No.: Ab118326

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, IHC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

OTI5E4

Conjugation

Unconjugated

Übersicht

Rabbit anti Human NME4 Antibody, Monoclonal (OTI5E4), could be used for WB, IHC, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

NME4 Mouse mAb - Primärer Antikörper, specific to NME4

Synonyme

NME4 | NDPK-D | NM23H4 | nm23-H4 | metastatic inhibition factor NM23H4 antibody | mitochondrial antibody | NDK antibody | NDKM_HUMAN antibody | NDP kinase antibody | NDP kinase D antibody | NDP kinase | NDPK D antibody | NDPKD antibody | nm23 H4 antibody

Spezifikationen & Reinheit

ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

Major role in the synthesis of nucleoside triphosphates other than ATP.

Wirtsspezies

Maus

Spezifität

NME4

Immunogen

Recombinant Full length protein of human NME4.

Positive Kontrolle

WB: Hep G2, A549 and Jurkat cell lysates. IHC: Human colon, kidney, liver, ovary tissue. IF/ICC: COS7 cells transiently transfected by pCMV6-ENTRY NME4. Flow Cytometry: HEK293T cells transfected with pCMV6-ENTRY NME4.

Konjugation

Unconjugated

Note

ExactAb™, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - Western Blot
All lanes: NME4 Mouse mAb (Ab118326) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 17 kDa
Exposure time: 71.5 s

Flow Cytometry: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - Flow Cytometry
HEK293T cells transfected with either pCMV6-ENTRY NME4(Red) or empty vector control plasmid (Blue) were immunostained with NME4 Mouse mAb (Ab118326), and then analyzed by flow cytometry.

IF/ICC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IF/ICC
NME4 Mouse mAb (Ab118326) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NME4.

IHC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of colon tissue using NME4 Mouse mAb (Ab118326). (Heat-induced epitope retrieval by 10-mM citric buffer, pH-6.0, 100°C for 10 min, Dilution 1/50).

IHC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IHC
Immunohistochemical staining of paraffinembedded Kidney tissue within the normal limits using NME4 Mouse mAb (Ab118326). (Heat-induced epitope retrieval by 10-mM citric buffer, pH-6.0, 100°C for 10 min, Dilution 1/50).

IHC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IHC
Immunohistochemical staining of paraffinembedded liver tissue within the normal limits using NME4 Mouse mAb (Ab118326). (Heat-induced epitope retrieval by 10-mM citric buffer, pH-6.0, 100°C for 10 min, Dilution 1/50).

IHC: NME4 Mouse mAb (Ab118326)

NME4 Mouse mAb (Ab118326) - IHC
Immunohistochemical staining of paraffinembedded Adenocarcinoma of ovary tissue using NME4 Mouse mAb (Ab118326). (Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 min, Dilution 1/50).

Anwendung

• Anwendung: Dilution info
• WB:

  1/2000 ;

• IF/ICC:

  1/100;

• Flow Cytometry:

  1/100;

• IHC:

  1/50

Zugehörige Ziele (menschlich)

NME4 Tbio Nucleoside diphosphate kinase, mitochondrial (0 Activities)

Discover Target: NME4

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (NME4 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.