OBFC1 Mouse mAb - Primärer Antikörper, specific to STN1

Cat. No.: Ab119117

GRADE & PURITY Validated, ExactAb™, See COA

Application

Flow Cytometry, IF/ICC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

OTI2E4

Conjugation

Unconjugated

Übersicht

Mouse anti Human OBFC1 Antibody, Monoclonal (OTI2E4), could be used for WB, IF/ICC, Flow Cytometry and so on.

Specifications

Product Name

OBFC1 Mouse mAb - Primärer Antikörper, specific to STN1

Synonyme

AAF 44 | AAF44 | Alpha accessory factor 44 | bA541N10.2 | CST complex subunit STN1 | FLJ22559 | Obfc1 | Oligonucleotide/oligosaccharide binding fold containing 1 | Oligonucleotide/oligosaccharide-binding fold-containing protein 1 | OTTHUMP00000020417 | OT

Spezifikationen & Reinheit

ExactAb™, Validated, See COA

Biochemische und physiologische Mechanismen

Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bin

Wirtsspezies

Maus

Spezifität

STN1

Immunogen

Recombinant protein of human OBFC1.

Positive Kontrolle

WB: Hep G2, A549, Jurkat and MCF7 celllysates. IF/ICC: COS7 transiently transfected with pCMV6-ENTRY OBFC. Flow Cytometry: HEK293T transfected with pCMV6-ENTRY OBFC1.

Konjugation

Unconjugated

Note

ExactAb™, Validated

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (12 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - Western Blot
All lanes: OBFC1 Mouse mAb (Ab119117) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 41 kDa
Exposure time: 100.0 s

IF/ICC: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - IF/ICC
OBFC1 Mouse mAb (Ab119117) immunofluorescent staining of COS7cells transiently transfected by pCMV6-ENTRYOBFC1.

Flow Cytometry​: OBFC1 Mouse mAb (Ab119117)

OBFC1 Mouse mAb (Ab119117) - Flow Cytometry​
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by OBFC1 Mouse mAb (Ab119117) , and then analyzed by flow cytometry.

Anwendung

• Anwendung: Dilution info
• WB:

  1/2000;

• Flow Cytometry:

  1/100;

• IF/ICC:

  1/100;

Zugehörige Ziele (menschlich)

STN1 Tbio CST complex subunit STN1 (0 Activities)

Discover Target: STN1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Validated mean?

Validated indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (OBFC1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.