GRADE & PURITYCarrier Free?Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.Azide Free?Azide-free — without sodium azide preservative. Use in conjugations, cell work, or assays where azide is toxic or inhibitory.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.See COA
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA Azide Free,Carrier Free,ExactAb™,High Performance,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Mouse anti Human p53 Antibody, Monoclonal (D1), could be used for WB and so on.
Application
WB: 0.01-2 µg/mL
Protein Function
Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over. Isoform 2 enhances the transactivation activity of isoform 1 from some but not all TP53-inducible promoters. Isoform 4 suppresses transactivation activity and impairs growth suppression mediated by isoform 1. Isoform 7 inhibits isoform 1-mediated apoptosis.
Specifications
Product Name
p53 Mouse mAb - Primary antibody, specific to p53, Mouse IgG1
Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA
Host species
Mouse
Specificity
p53
Immunogen
Recombinant p53 expressed in E.coli (AA 133-393).
Positive Control
WB: HEK293, MCF7, HT-29, HeLa, A549 and C6 cell lysates.
Conjugation
Unconjugated
Grade
Azide Free, Carrier Free, ExactAb™, High Performance, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein A + Protein G purified
Concentration
See COA
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images
p53 Mouse mAb (Ab119827) - Western Blot All lanes: p53 Mouse mAb (Ab119827) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution
Predicted band size: 53 kDa Observed band size: 50 kDa Exposure time: 29.1 s
Application
Application
Dilution info
WB
0.01-2 µg/mL
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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