PAI1 Mouse mAb - Primärer Antikörper, specific to SERPINE1, Mouse IgG1

Cat. No.: Ab120074

GRADE & PURITY Carrier Free, Validated, ExactAb™, Azide Free, High Performance, See COA

Application

IF/ICC, IHC, WB

Host species

Maus

Reactive species

Mensch

Isotype

Mouse IgG1

Clone Number

J11

Conjugation

Unconjugated

Übersicht

Mouse anti Human PAI1 Antibody, Monoclonal(J11), could be used for WB, IHC, IF, ICC and so on .

Application:

WB: 0.01-2µg/mL IHC: 5-20µg/mL IF/ICC: 5-20µg/mL

Protein Function

This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis.

Specifications

Product Name

PAI1 Mouse mAb - Primärer Antikörper, specific to SERPINE1, Mouse IgG1

Synonyme

Endothelial plasminogen activator inhibitor | Nexin | PAI1 | PAI-1 | PAI1PAI-1 | PAISerpin E1 | PLANH1 | PLANH1plasminogen activator inhibitor 1 | serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogenactivator inhibitor type 1), member 1

Spezifikationen & Reinheit

Carrier Free, ExactAb™, Azide Free, Validated, High Performance, See COA

Wirtsspezies

Maus

Spezifität

SERPINE1

Immunogen

Recombinant PAI1 expressed in E.coli (AA 24-402).

Positive Kontrolle

WB: HeLa cell lysate. IHC: Human kidney tissue. IF/ICC: HeLa cells.

Konjugation

Unconjugated

Note

Azide Free, Carrier Free, ExactAb™, High Performance, Validated

Isotyp

Mouse IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein A + Protein G purified

Konzentration

See COA

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Store at 4℃ short term (1-2 weeks). Store at -20℃ long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Bilder

Western Blot: PAI1 Mouse mAb (Ab120074)

PAI1 Mouse mAb (Ab120074) - Western Blot
All lanes: PAI1 Mouse mAb (Ab120074) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 45 kDa
Observed band size: 47 kDa
Exposure time: 20.5 s

IF/ICC: PAI1 Mouse mAb (Ab120074)

PAI1 Mouse mAb (Ab120074) - IF/ICC
FITC staining on IF
Sample: Human HeLa cells
Primary Ab: 20 μg/mL PAI1 Mouse mAb (Ab120074)
Second Ab: 5 μg/mL FITC-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

IHC: PAI1 Mouse mAb (Ab120074)

PAI1 Mouse mAb (Ab120074) - IHC
DAB staining on IHC
Sample: Human Kidney Tissue
Primary Ab: 20 μg/mL PAI1 Mouse mAb (Ab120074)
Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody.

Anwendung

• Anwendung: Dilution info
• IHC:

  5-20 µg/mL;

• WB:

  0.01-2 µg/mL ;

• IF/ICC:

  5-20µg/mL;

Zugehörige Ziele (menschlich)

SERPINE1 Tchem Plasminogen activator inhibitor 1 (0 Activities)

Discover Target: SERPINE1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PAI1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.