PDHA1 Mouse mAb - Primary antibody, specific to PDHA1, Mouse IgG1

Cat. No.: Ab120952
AVAILABLE TO ORDER
GRADE & PURITY Validated ? Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated. ExactAb™ ? ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications. 1.14 mg/mL
Application
IF/ICC, WB
Host species
Mouse
Reactive species
Human, Mouse, Rat
Isotype
Mouse IgG1
Clone Number
3H2-F8-B5
Conjugation
Unconjugated
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
10μl
Ab120952-10μl
2
$79.90
50μl
Ab120952-50μl
1
$259.90
100μl
Ab120952-100μl
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$419.90
1ml
Ab120952-1ml
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$3,399.90
Enter a quantity for the sizes you want to add.
🧪

Why this grade

ExactAb™, Validated, 1.14 mg/mL ExactAb™,Validated for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Overview

Mouse anti Human PDHA1 Antibody, Monoclonal (3H2-F8-B5), could be used for WB, ICC, IF and so on.
Application:
WB: 1/500-1/1000
ICC/IF: 1/50-1/200
Protein Function
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3).

Specifications

Product Name
PDHA1 Mouse mAb - Primary antibody, specific to PDHA1, Mouse IgG1
Synonyms
ODPA_HUMAN antibody | PDH antibody | PDHA antibody | PDHA1 antibody | PDHCE1A antibody | PDHE1 A type Iantibody | PDHE1-A type I antibody | PHE1A antibody | Pyruvate Dehydrogenase (lipoamide) alpha 1 antibody | Pyruvate dehydrogenase complex, E1 alpha pol
Specifications & Purity
ExactAb™, Validated, 1.14 mg/mL
Host species
Mouse
Specificity
PDHA1
Immunogen
Purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli (AA 131-389).
Positive Control
WB: HeLa, HEK293, Hep G2, A549, NIH/3T3 and C6 cell lysates. ICC/IF: HeLa cells.
Conjugation
Unconjugated
Grade
ExactAb™, Validated
Isotype
Mouse IgG1
Light Chain Type
kappa
SDS-PAGE
150 kDa
Purification Method
Protein G purified
Concentration
1.14 mg/mL
Storage and Shipping
Shape
Liquid
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at 4°C short term (1-2 weeks). Store at -20°C long term (24 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Images

PDHA1 Mouse mAb (Ab120952) - Western Blot
All lanes: PDHA1 Mouse mAb (Ab120952) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 43 kDa
Observed band size: 41 kDa
Exposure time: 20.0 s


PDHA1 Mouse mAb (Ab120952) - IF/ICC
Immunocytochemistry analysis of Pyruvate Dehydrogenase E1 alpha in HeLa using PDHA1 Mouse mAb (Ab120952).

Application
ApplicationDilution info
WB

1/500-1/1000

IF/ICC

1/50-1/200

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Associated Targets(Human)
PDHA1 Tbio Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial (0 Activities)
Activity TypeActivity Value -log(M)Mechanism of ActionActivity ReferencePublications (PubMed IDs)
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

5 results found

Lot NumberCertificate TypeDateItem
ZJ26F0636173Certificate of AnalysisJun 08, 2026 Ab120952
ZJ26F0636172Certificate of AnalysisJun 08, 2026 Ab120952
ZJ24F0607376Certificate of AnalysisApr 07, 2026 Ab120952
ZJ23F1202126Certificate of AnalysisDec 20, 2023 Ab120952
ZJ23F1202125Certificate of AnalysisDec 20, 2023 Ab120952
Documents & Articles
Solution Calculators
Reviews

Customer Reviews

Need help choosing the grade?

Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.

View Validated grade guide → View ExactAb™ grade guide →

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