PDPK1 Mouse mAb - Primärer Antikörper, specific to PDPK1, IgG1

Cat. No.: Ab121091

GRADE & PURITY Carrier Free, Validated, ExactAb™, 1.0mg/mL

Application

ELISA, Flow Cytometry, IF/ICC, WB

Host species

Maus

Reactive species

Maus, Mensch, Rat

Isotype

Mouse IgG1

Clone Number

3H3D9

Conjugation

Unconjugated

Übersicht

Application:
WB: 1/500 - 1/2000. Predicted molecular weight: 63 kDa.
IF/ICC: 1/200 - 1/1000
Flow: 1/200 - 1/400
ELISA: 1/10000

Protein Function
Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development (By similarity). Isoform 3 is catalytically inactive.

Specifications

Product Name

PDPK1 Mouse mAb - Primärer Antikörper, specific to PDPK1, IgG1

Synonyme

EC 2.7.11.1 | hPDK1

Spezifikationen & Reinheit

ExactAb™, Validated, Carrier Free, 1.0mg/mL

Wirtsspezies

Maus

Spezifität

PDPK1

Immunogen

Purified recombinant fragment of human PDPK1 (AA: 457-556) expressed in E. Coli.

Positive Kontrolle

WB: C6, K562, NIH/3T3 and MCF7 cell lysates. Flow: HeLa cells. ICC/IF: HeLa cells.

Konjugation

Unconjugated

Note

Carrier Free, ExactAb™, Validated

Isotyp

IgG1

Lichterketten-Typ

kappa

SDS-PAGE

150 kDa

Verfahren zur Reinigung

Protein G purified

Konzentration

1.0mg/mL

Lagerung und Versand

Form

Liquid

Storage

Store at -20°C,Avoid repeated freezing and thawing

Verschickt in

Ice chest + Ice pads

Stabilität und Lagerung

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon receipt, it is recommended to aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

Bilder

Western Blot: PDPK1 Mouse mAb (Ab121091)

PDPK1 Mouse mAb (Ab121091) - Western Blot
All lanes: PDPK1 Mouse mAb (Ab121091) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/20000 dilution

Predicted band size: 64 kDa
Observed band size: 51- 64 kDa
Exposure time: 113.7 s

ICC/IF: PDPK1 Mouse mAb (Ab121091)

PDPK1 Mouse mAb (Ab121091) - ICC/IF
Immunofluorescence analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with AF555 phalloidin.

Flow Cytometry: PDPK1 Mouse mAb (Ab121091)

PDPK1 Mouse mAb (Ab121091) - Flow Cytometry
Flow cytometric analysis of HeLa cells using PDPK1 Mouse mAb (Ab121091) (green) and negative control (red).

Anwendung

• Anwendung: Dilution info
• IF/ICC:

  1/200-1/1000 ;

• Flow Cytometry:

  1/200-1/400 ;

• ELISA:

  1/10000 ;

Zugehörige Ziele (menschlich)

PDPK1 Tchem 3-phosphoinositide-dependent protein kinase 1 (0 Activities)

Discover Target: PDPK1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)

Wirkungsmechanismen

Häufig gestellte Fragen

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

What does ExactAb? mean?

ExactAb? indicates the product has been validated for use in rigorous antibody validation workflows. Validation may include positive/negative control samples, signal-to-noise testing, and protocol-specific QC. Lot-specific test data is published on the Certificate of Analysis where available.

What does Carrier Free mean?

Carrier Free indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.

Which species reactivity has this antibody been validated against?

Validated reactivity is listed on the PDP and on each lot's datasheet. We validate against the most commonly tested species for the target (PDPK1 Mouse mAb); cross-reactivity in untested species may exist but is not guaranteed. Contact technical support for specific species inquiries.